Enhancement of anti-Aeromonas salmonicida activity in Atlantic salmon (Salmo salar) macrophages by a mannose-binding lectin

Christopher A. Ottinger, Stewart C. Johnson, K. Vanya Ewart, Laura L. Brown, Neil W. Ross

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

82 Citas (Scopus)

Resumen

We investigated the effects of a calcium-dependent mannose-binding lectin isolated from the serum of Atlantic salmon on Aeromonas salmonicida viability and the anti-A. salmonicida activity of Atlantic salmon macrophages. In the absence of other factors, binding of this lectin at concentrations of 0.8, 4.0 and 20.0 ng ml-1 to virulent A. salmonicida failed to significantly reduce (P>0.05) cell viability. However, binding of the lectin to A. salmonicida did result in significant (P≤0.05) dose-dependent increases in phagocytosis, and bactericidal activity. Significant increases (P≤0.05) were also observed in phagocyte respiratory burst activity within the lectin concentration range of 4.0-20.0 ng ml-1 but the stimulation was not dose dependent at these lectin concentrations. At the lowest lectin concentration tested (0.32 ng ml-1), a significant decrease (P≤0.05) in respiratory burst was observed. The structure and activity of this lectin are similar to that of mammalian mannose-binding lectins, which are known to play a pivotal role in innate immunity. The presence of this lectin may be an important defense mechanism against Gram-negative bacteria such as A. salmonicida. Copyright (C) 1999 Elsevier Science Inc.

Idioma originalEnglish
Páginas (desde-hasta)53-59
Número de páginas7
PublicaciónComparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology
Volumen123
N.º1
DOI
EstadoPublished - may. 1999
Publicado de forma externa

Nota bibliográfica

Funding Information:
We thank Dr Michael Reith for critically reviewing this manuscript. This research was funded by the Institute for Marine Biosciences, National Research Council, Canada. C.A.O. was supported by a NSERC Fellowship.

ASJC Scopus Subject Areas

  • Immunology
  • Pharmacology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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