Evidence that the fragmented ribosomal RNAs of Chlamydomonas mitochondria are associated with ribosomes

Eileen M. Denovan-Wright; Robert W. Lee

doi:10.1016/0014-5793(95)00837-Y

Evidence that the fragmented ribosomal RNAs of Chlamydomonas mitochondria are associated with ribosomes

Eileen M. Denovan-Wright, Robert W. Lee

Medicine

Medicine

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12 Citas (Scopus)

Resumen

The discontinuous and scrambled organization of the small subunit and large subunit rRNA coding regions in Chlamydomonas mitochondrial DNA has been well documented. Our goals were to demonstrate that the small transcripts produced by these coding regions in Chlamydomonas eugametos are assembled into mitochondrial ribosomes and to characterize the sedimentation properties of these ribosomes and their subunits in sucrose gradients. Putative mitochondrial ribosomes (60-66S) and their large (44-50S) and small (35-39S) subunits were identified by slot blot hybridization which sedimented independently of the chloroplast and cytosolic ribosomes. A crude mitochondrial pellet prepared from C. eugametos was enriched for mitochondrial small subunit and large subunit rRNA subfragments thereby providing independent confirmation of the mitochondrial association of these rRNA molecules.

Idioma original	English
Páginas (desde-hasta)	222-226
Número de páginas	5
Publicación	FEBS Letters
Volumen	370
N.º	3
DOI	https://doi.org/10.1016/0014-5793(95)00837-Y
Estado	Published - ago. 21 1995

Nota bibliográfica

Funding Information:
Acknowledgements: This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to R.W. Lee. E. Denovan-Wright was an Izaak Walton Killam Memorial Scholar and held an NSERC post-graduate scholarship. We thank M.N. Schnare and D.F. Spencer for helpful discussions.

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Evidence that the fragmented ribosomal RNAs of Chlamydomonas mitochondria are associated with ribosomes",

abstract = "The discontinuous and scrambled organization of the small subunit and large subunit rRNA coding regions in Chlamydomonas mitochondrial DNA has been well documented. Our goals were to demonstrate that the small transcripts produced by these coding regions in Chlamydomonas eugametos are assembled into mitochondrial ribosomes and to characterize the sedimentation properties of these ribosomes and their subunits in sucrose gradients. Putative mitochondrial ribosomes (60-66S) and their large (44-50S) and small (35-39S) subunits were identified by slot blot hybridization which sedimented independently of the chloroplast and cytosolic ribosomes. A crude mitochondrial pellet prepared from C. eugametos was enriched for mitochondrial small subunit and large subunit rRNA subfragments thereby providing independent confirmation of the mitochondrial association of these rRNA molecules.",

author = "Denovan-Wright, {Eileen M.} and Lee, {Robert W.}",

note = "Funding Information: Acknowledgements: This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to R.W. Lee. E. Denovan-Wright was an Izaak Walton Killam Memorial Scholar and held an NSERC post-graduate scholarship. We thank M.N. Schnare and D.F. Spencer for helpful discussions.",

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AU - Denovan-Wright, Eileen M.

AU - Lee, Robert W.

N1 - Funding Information: Acknowledgements: This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to R.W. Lee. E. Denovan-Wright was an Izaak Walton Killam Memorial Scholar and held an NSERC post-graduate scholarship. We thank M.N. Schnare and D.F. Spencer for helpful discussions.

PY - 1995/8/21

Y1 - 1995/8/21

N2 - The discontinuous and scrambled organization of the small subunit and large subunit rRNA coding regions in Chlamydomonas mitochondrial DNA has been well documented. Our goals were to demonstrate that the small transcripts produced by these coding regions in Chlamydomonas eugametos are assembled into mitochondrial ribosomes and to characterize the sedimentation properties of these ribosomes and their subunits in sucrose gradients. Putative mitochondrial ribosomes (60-66S) and their large (44-50S) and small (35-39S) subunits were identified by slot blot hybridization which sedimented independently of the chloroplast and cytosolic ribosomes. A crude mitochondrial pellet prepared from C. eugametos was enriched for mitochondrial small subunit and large subunit rRNA subfragments thereby providing independent confirmation of the mitochondrial association of these rRNA molecules.

AB - The discontinuous and scrambled organization of the small subunit and large subunit rRNA coding regions in Chlamydomonas mitochondrial DNA has been well documented. Our goals were to demonstrate that the small transcripts produced by these coding regions in Chlamydomonas eugametos are assembled into mitochondrial ribosomes and to characterize the sedimentation properties of these ribosomes and their subunits in sucrose gradients. Putative mitochondrial ribosomes (60-66S) and their large (44-50S) and small (35-39S) subunits were identified by slot blot hybridization which sedimented independently of the chloroplast and cytosolic ribosomes. A crude mitochondrial pellet prepared from C. eugametos was enriched for mitochondrial small subunit and large subunit rRNA subfragments thereby providing independent confirmation of the mitochondrial association of these rRNA molecules.

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Evidence that the fragmented ribosomal RNAs of Chlamydomonas mitochondria are associated with ribosomes

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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