Exposure of breast cancer cells to a subcytotoxic dose of apigenin causes growth inhibition, oxidative stress, and hypophosphorylation of Akt

Megan E. Harrison, Melanie R. Power Coombs, Leanne M. Delaney, David W. Hoskin

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

63 Citas (Scopus)

Resumen

Epidemiological studies show that fruit- and vegetable-rich diets are associated with a reduced risk of developing certain forms of cancer, including breast cancer. In this study we demonstrate that a subcytotoxic concentration of apigenin, which is a flavone found at high concentrations in parsley, onions, grapefruit, oranges, and chamomile tea, inhibited DNA synthesis in a panel of human breast cancer cell lines (MDA-MB-231, MBA-MB-468, MCF-7, SK-BR-3). Decreased proliferation of MDA-MB-468 cells in the presence of apigenin was associated with G2/M phase cell cycle arrest and the production of reactive oxygen species. Apigenin-treated MDA-MB-468 cells also showed reduced phosphorylation of Akt (protein kinase B), which is an essential effector serine/threonine kinase in the phosphatidylinositide 3-kinase pathway that promotes tumor growth and progression. However, exposure to the antioxidant reduced glutathione failed to reverse apigenin-mediated inhibition of Akt phosphorylation and cell proliferation, indicating that these effects were not due to oxidative stress. Taken together, these findings suggest that low-dose apigenin has the potential to slow or prevent breast cancer progression.

Idioma originalEnglish
Páginas (desde-hasta)211-217
Número de páginas7
PublicaciónExperimental and Molecular Pathology
Volumen97
N.º2
DOI
EstadoPublished - oct. 2014

Nota bibliográfica

Funding Information:
The work was supported by the Dalhousie Medical Research Foundation and the Canadian Breast Cancer Foundation Atlantic Region . Megan Harrison was the recipient of a Frederick Banting and Charles Best Canada Graduate Scholarship, a Canadian Imperial Bank of Commerce Graduate Scholarship in Medical Research and a trainee award from The Beatrice Hunter Cancer Research Institute as part of the Terry Fox Strategic Health Research Training Program in Cancer Research at the Canadian Institutes of Health Research.

ASJC Scopus Subject Areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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