Gene-expression analysis after alcohol exposure in the developing mouse

Marjie L. Hard; Mohamed Abdolell; Brian H. Robinson; Gideon Koren

doi:10.1016/j.lab.2004.11.011

Gene-expression analysis after alcohol exposure in the developing mouse

Marjie L. Hard, Mohamed Abdolell, Brian H. Robinson, Gideon Koren

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58 Citas (Scopus)

Resumen

Exposure to alcohol in the embryonic mouse can lead to structural and neurophysiologic changes. The cause of these changes is poorly understood, but they are likely the result of numerous mechanisms. Here we investigate ethanol-induced alterations in gene expression in the fetal brain. Using complementary-DNA microarrays, we identified 25 genes that were down-regulated by prenatal ethanol exposure on days 7 and 9 of gestation. None were found to be up-regulated. Of those that were repressed, 6 (Timp4, Bmp15, Rnf25, Akt1, Tulp4, Dexras1) have been identified, and they are discussed here in the context of the developing fetus. The identified genes have been shown to be involved in cell proliferation, differentiation, and apoptosis, and they contribute to tissue growth and remodeling, as well as neuronal growth and survival. Microarray studies may be useful in the identification of a genetic marker for fetal alcohol syndrome, the discovery of novel pathways that may be involved in its origin, or both.

Idioma original	English
Páginas (desde-hasta)	47-54
Número de páginas	8
Publicación	Journal of Laboratory and Clinical Medicine
Volumen	145
N.º	1
DOI	https://doi.org/10.1016/j.lab.2004.11.011
Estado	Published - ene. 2005
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Supported by a grant from the Canadian Institute of Health Research. Dr Koren is a senior scientist of the Canadian Institute of Health Research and a holder of the Ivey Chair in Molecular Toxicology, The University of Western Ontario. Ms Hard is a recipient of a graduate studentship from the Research Institute of The Hospital for Sick Children and the Ontario Graduate Scholarship.

ASJC Scopus Subject Areas

Pathology and Forensic Medicine

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title = "Gene-expression analysis after alcohol exposure in the developing mouse",

abstract = "Exposure to alcohol in the embryonic mouse can lead to structural and neurophysiologic changes. The cause of these changes is poorly understood, but they are likely the result of numerous mechanisms. Here we investigate ethanol-induced alterations in gene expression in the fetal brain. Using complementary-DNA microarrays, we identified 25 genes that were down-regulated by prenatal ethanol exposure on days 7 and 9 of gestation. None were found to be up-regulated. Of those that were repressed, 6 (Timp4, Bmp15, Rnf25, Akt1, Tulp4, Dexras1) have been identified, and they are discussed here in the context of the developing fetus. The identified genes have been shown to be involved in cell proliferation, differentiation, and apoptosis, and they contribute to tissue growth and remodeling, as well as neuronal growth and survival. Microarray studies may be useful in the identification of a genetic marker for fetal alcohol syndrome, the discovery of novel pathways that may be involved in its origin, or both.",

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N2 - Exposure to alcohol in the embryonic mouse can lead to structural and neurophysiologic changes. The cause of these changes is poorly understood, but they are likely the result of numerous mechanisms. Here we investigate ethanol-induced alterations in gene expression in the fetal brain. Using complementary-DNA microarrays, we identified 25 genes that were down-regulated by prenatal ethanol exposure on days 7 and 9 of gestation. None were found to be up-regulated. Of those that were repressed, 6 (Timp4, Bmp15, Rnf25, Akt1, Tulp4, Dexras1) have been identified, and they are discussed here in the context of the developing fetus. The identified genes have been shown to be involved in cell proliferation, differentiation, and apoptosis, and they contribute to tissue growth and remodeling, as well as neuronal growth and survival. Microarray studies may be useful in the identification of a genetic marker for fetal alcohol syndrome, the discovery of novel pathways that may be involved in its origin, or both.

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Gene-expression analysis after alcohol exposure in the developing mouse

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

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