Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar

Isa N. Cruz, Conor S. Barry, Holger B. Kramer, C. Celeste Chuang, Sarah Lloyd, Aarnoud C. Van der Spoel, Frances M. Platt, Min Yang, Benjamin G. Davis

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

6 Citas (Scopus)

Resumen

Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the ‘glyco-interactome’ has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (Glyco-AeP) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and the associated genomic analysis implicate a subset of chaperone and junctional proteins as important in male fertility. Such repurposing of existing therapeutics thus creates direct routes to probing in vivo function. The success of this strategy suggests a general approach to discovering ‘carbohydrate-active’ partners in biology.

Idioma originalEnglish
Páginas (desde-hasta)3442-3446
Número de páginas5
PublicaciónChemical Science
Volumen4
N.º9
DOI
EstadoPublished - jul. 29 2013
Publicado de forma externa

ASJC Scopus Subject Areas

  • General Chemistry

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