Identification of radial glia-like cells in the adult mouse olfactory bulb

Jason G. Emsley; João R.L. Menezes; Rodrigo F. Madeiro Da Costa; Ana Maria Blanco Martinez; Jeffrey D. Macklis

doi:10.1016/j.expneurol.2012.05.012

Identification of radial glia-like cells in the adult mouse olfactory bulb

Jason G. Emsley, João R.L. Menezes, Rodrigo F. Madeiro Da Costa, Ana Maria Blanco Martinez, Jeffrey D. Macklis

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

5 Citas (Scopus)

Resumen

Immature neurons migrate tangentially within the rostral migratory stream (RMS) to the adult olfactory bulb (OB), then radially to their final positions as granule and periglomerular neurons; the controls over this transition are not well understood. Using adult transgenic mice with the human GFAP promoter driving expression of enhanced GFP, we identified a population of radial glia-like cells that we term adult olfactory radial glia-like cells (AORGs). AORGs have large, round somas and simple, radially oriented processes. Confocal reconstructions indicate that AORGs variably express typical radial glial markers, only rarely express mouse GFAP, and do not express astroglial, oligodendroglial, neuronal, or tanycyte markers. Electron microscopy provides further supporting evidence that AORGs are not immature neurons. Developmental analyses indicate that AORGs are present as early as P1, and are generated through adulthood. Tracing studies show that AORGs are not born in the SVZa, suggesting that they are born either in the RMS or the OB. Migrating immature neurons from the adult SVZa are closely apposed to AORGs during radial migration in vivo and in vitro. Taken together, these data indicate a newly-identified population of radial glia-like cells in the adult OB that might function uniquely in neuronal radial migration during adult OB neurogenesis.

Idioma original	English
Páginas (desde-hasta)	283-297
Número de páginas	15
Publicación	Experimental Neurology
Volumen	236
N.º	2
DOI	https://doi.org/10.1016/j.expneurol.2012.05.012
Estado	Published - ago. 2012

Nota bibliográfica

Funding Information:
We thank Alex Eswar, Kyle MacQuarrie, Ashley Palmer, Karen Billmers, and Aaron Wheeler for excellent technical assistance, and other members of the Macklis laboratory for their many helpful comments. The transgenic hGFAP-eGFP mouse line was a generous gift from Drs. Helmut Kettenmann and Christiane Nolte (Max Delbrück Center for Molecular Medicine, Berlin). We thank Dr. Tatjana Jakobs (MGH, HMS) for sharing her expertise with videomicroscopy, and Dr. Richard Masland (MGH, HMS) for the use of the videomicroscopy equipment. We thank Dr. Nathaniel Heintz (Rockefeller University) for the gift of BLBP antibody; Dr. Pam Follett (Children's Hospital, Boston) for the gift of A2B5 antibody; and Dr. Ron McKay (NIH) for the gift of the nestin antibody. We thank Carol Birmingham and Chemicon/Millipore for the gift of several antibodies. Several monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City. This work was supported by NIH grants NS45523 and NS49553 (to J.D.M.), with additional infrastructure provided by NS41590 (to J.D.M); grants from the Children's Neurobiological Solutions Foundation and United Sydney Foundation (to J.D.M.); and CNPq-Brazil and an NIH-NINDS International Fellowship (to J.R.L.M.). J.G.E. was partially supported by fellowships from the Heart and Stroke Foundation of Canada , the Paralyzed Veterans of America/Travis Roy Foundation , and the Curry Endowment of the Dalhousie Medical Research Foundation .

ASJC Scopus Subject Areas

Neurology
Developmental Neuroscience

Acceder al documento

10.1016/j.expneurol.2012.05.012

Otros archivos y enlaces

Citar esto

@article{be5f59ac38c84026a4398567ce20b522,

title = "Identification of radial glia-like cells in the adult mouse olfactory bulb",

abstract = "Immature neurons migrate tangentially within the rostral migratory stream (RMS) to the adult olfactory bulb (OB), then radially to their final positions as granule and periglomerular neurons; the controls over this transition are not well understood. Using adult transgenic mice with the human GFAP promoter driving expression of enhanced GFP, we identified a population of radial glia-like cells that we term adult olfactory radial glia-like cells (AORGs). AORGs have large, round somas and simple, radially oriented processes. Confocal reconstructions indicate that AORGs variably express typical radial glial markers, only rarely express mouse GFAP, and do not express astroglial, oligodendroglial, neuronal, or tanycyte markers. Electron microscopy provides further supporting evidence that AORGs are not immature neurons. Developmental analyses indicate that AORGs are present as early as P1, and are generated through adulthood. Tracing studies show that AORGs are not born in the SVZa, suggesting that they are born either in the RMS or the OB. Migrating immature neurons from the adult SVZa are closely apposed to AORGs during radial migration in vivo and in vitro. Taken together, these data indicate a newly-identified population of radial glia-like cells in the adult OB that might function uniquely in neuronal radial migration during adult OB neurogenesis.",

author = "Emsley, {Jason G.} and Menezes, {Jo{\~a}o R.L.} and {Madeiro Da Costa}, {Rodrigo F.} and Martinez, {Ana Maria Blanco} and Macklis, {Jeffrey D.}",

note = "Funding Information: We thank Alex Eswar, Kyle MacQuarrie, Ashley Palmer, Karen Billmers, and Aaron Wheeler for excellent technical assistance, and other members of the Macklis laboratory for their many helpful comments. The transgenic hGFAP-eGFP mouse line was a generous gift from Drs. Helmut Kettenmann and Christiane Nolte (Max Delbr{\"u}ck Center for Molecular Medicine, Berlin). We thank Dr. Tatjana Jakobs (MGH, HMS) for sharing her expertise with videomicroscopy, and Dr. Richard Masland (MGH, HMS) for the use of the videomicroscopy equipment. We thank Dr. Nathaniel Heintz (Rockefeller University) for the gift of BLBP antibody; Dr. Pam Follett (Children's Hospital, Boston) for the gift of A2B5 antibody; and Dr. Ron McKay (NIH) for the gift of the nestin antibody. We thank Carol Birmingham and Chemicon/Millipore for the gift of several antibodies. Several monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City. This work was supported by NIH grants NS45523 and NS49553 (to J.D.M.), with additional infrastructure provided by NS41590 (to J.D.M); grants from the Children's Neurobiological Solutions Foundation and United Sydney Foundation (to J.D.M.); and CNPq-Brazil and an NIH-NINDS International Fellowship (to J.R.L.M.). J.G.E. was partially supported by fellowships from the Heart and Stroke Foundation of Canada , the Paralyzed Veterans of America/Travis Roy Foundation , and the Curry Endowment of the Dalhousie Medical Research Foundation .",

year = "2012",

month = aug,

doi = "10.1016/j.expneurol.2012.05.012",

language = "English",

volume = "236",

pages = "283--297",

journal = "Experimental Neurology",

issn = "0014-4886",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Identification of radial glia-like cells in the adult mouse olfactory bulb

AU - Emsley, Jason G.

AU - Menezes, João R.L.

AU - Madeiro Da Costa, Rodrigo F.

AU - Martinez, Ana Maria Blanco

AU - Macklis, Jeffrey D.

N1 - Funding Information: We thank Alex Eswar, Kyle MacQuarrie, Ashley Palmer, Karen Billmers, and Aaron Wheeler for excellent technical assistance, and other members of the Macklis laboratory for their many helpful comments. The transgenic hGFAP-eGFP mouse line was a generous gift from Drs. Helmut Kettenmann and Christiane Nolte (Max Delbrück Center for Molecular Medicine, Berlin). We thank Dr. Tatjana Jakobs (MGH, HMS) for sharing her expertise with videomicroscopy, and Dr. Richard Masland (MGH, HMS) for the use of the videomicroscopy equipment. We thank Dr. Nathaniel Heintz (Rockefeller University) for the gift of BLBP antibody; Dr. Pam Follett (Children's Hospital, Boston) for the gift of A2B5 antibody; and Dr. Ron McKay (NIH) for the gift of the nestin antibody. We thank Carol Birmingham and Chemicon/Millipore for the gift of several antibodies. Several monoclonal antibodies were obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City. This work was supported by NIH grants NS45523 and NS49553 (to J.D.M.), with additional infrastructure provided by NS41590 (to J.D.M); grants from the Children's Neurobiological Solutions Foundation and United Sydney Foundation (to J.D.M.); and CNPq-Brazil and an NIH-NINDS International Fellowship (to J.R.L.M.). J.G.E. was partially supported by fellowships from the Heart and Stroke Foundation of Canada , the Paralyzed Veterans of America/Travis Roy Foundation , and the Curry Endowment of the Dalhousie Medical Research Foundation .

PY - 2012/8

Y1 - 2012/8

N2 - Immature neurons migrate tangentially within the rostral migratory stream (RMS) to the adult olfactory bulb (OB), then radially to their final positions as granule and periglomerular neurons; the controls over this transition are not well understood. Using adult transgenic mice with the human GFAP promoter driving expression of enhanced GFP, we identified a population of radial glia-like cells that we term adult olfactory radial glia-like cells (AORGs). AORGs have large, round somas and simple, radially oriented processes. Confocal reconstructions indicate that AORGs variably express typical radial glial markers, only rarely express mouse GFAP, and do not express astroglial, oligodendroglial, neuronal, or tanycyte markers. Electron microscopy provides further supporting evidence that AORGs are not immature neurons. Developmental analyses indicate that AORGs are present as early as P1, and are generated through adulthood. Tracing studies show that AORGs are not born in the SVZa, suggesting that they are born either in the RMS or the OB. Migrating immature neurons from the adult SVZa are closely apposed to AORGs during radial migration in vivo and in vitro. Taken together, these data indicate a newly-identified population of radial glia-like cells in the adult OB that might function uniquely in neuronal radial migration during adult OB neurogenesis.

AB - Immature neurons migrate tangentially within the rostral migratory stream (RMS) to the adult olfactory bulb (OB), then radially to their final positions as granule and periglomerular neurons; the controls over this transition are not well understood. Using adult transgenic mice with the human GFAP promoter driving expression of enhanced GFP, we identified a population of radial glia-like cells that we term adult olfactory radial glia-like cells (AORGs). AORGs have large, round somas and simple, radially oriented processes. Confocal reconstructions indicate that AORGs variably express typical radial glial markers, only rarely express mouse GFAP, and do not express astroglial, oligodendroglial, neuronal, or tanycyte markers. Electron microscopy provides further supporting evidence that AORGs are not immature neurons. Developmental analyses indicate that AORGs are present as early as P1, and are generated through adulthood. Tracing studies show that AORGs are not born in the SVZa, suggesting that they are born either in the RMS or the OB. Migrating immature neurons from the adult SVZa are closely apposed to AORGs during radial migration in vivo and in vitro. Taken together, these data indicate a newly-identified population of radial glia-like cells in the adult OB that might function uniquely in neuronal radial migration during adult OB neurogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84861949307&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861949307&partnerID=8YFLogxK

U2 - 10.1016/j.expneurol.2012.05.012

DO - 10.1016/j.expneurol.2012.05.012

M3 - Article

C2 - 22634209

AN - SCOPUS:84861949307

SN - 0014-4886

VL - 236

SP - 283

EP - 297

JO - Experimental Neurology

JF - Experimental Neurology

IS - 2

ER -

Identification of radial glia-like cells in the adult mouse olfactory bulb

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto