Immunohistochemical expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the normal human kidney development

Anita Racetin, Fila Raguž, Merica Glavina Durdov, Nenad Kunac, Marijan Saraga, Simone Sanna-Cherchi, Violeta Šoljić, Vlatka Martinović, Joško Petričević, Sandra Kostić, Snježana Mardešić, Sandra Zekić Tomaš, Boris Kablar, Ivana Restović, Mirela Lozić, Natalija Filipović, Mirna Saraga-Babić, Katarina Vukojević

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

16 Citas (Scopus)

Resumen

Aim: Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling. Methods: Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal–Wallis test. Results: In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p < 0.05). RIP5/FGFR1 co-localized at the marginal zone and the ureteric bud with predominant FGFR1 expression. FGFR2 (26.1%) shows similar expression pattern as FGFR1 (70.5%) in the same kidney structures. RIP5/FGFR2 co-localized at the marginal zone and the collecting ducts (predominant expression of FGFR2). HIP2 is strongly expressed in collecting ducts (96.7%), and co-localized with RIP5. In 10th week, RIP5 expression decrease (74.2%), while the pattern of expression of RIP5 and FGFR1 in collecting ducts (33.4% and 91.9%) and developing nephrons (21.9% and 32.4%) (p < 0.05) is similar to that in the 6th developmental week. Ureter is moderately expressing RIP5 while FGFR1 is strongly expressed in the ureteric wall. FGFR2 is strongly expressed in the collecting ducts (84.3%) and ureter. HIP2 have 81.1% positive cells in the collecting duct. RIP5/FGFR1 co-localize in collecting ducts and Henley's loop. Conclusions: The expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the human kidney development might indicate their important roles in metanephric development and ureteric muscle layer differentiation through FGF signaling pathways.

Idioma originalEnglish
Páginas (desde-hasta)531-538
Número de páginas8
PublicaciónActa Histochemica
Volumen121
N.º5
DOI
EstadoPublished - jul. 2019

Nota bibliográfica

Funding Information:
This work was supported by the Croatian Science Foundation (grant no. IP-06-2016-2575 ); splitsko-dalmatinska županija 2017 . “Capacity building for research development and kidney disease diagnosis“.

Publisher Copyright:
© 2019 Elsevier GmbH

ASJC Scopus Subject Areas

  • Histology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article

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