Immunostimulatory principles from Chlorella pyrenoidosa-Part 1: Isolation and biological assessment in vitro

J. A. Kralovec; K. L. Metera; J. R. Kumar; L. V. Watson; G. S. Girouard; Y. Guan; R. I. Carr; C. J. Barrow; H. S. Ewart

doi:10.1016/j.phymed.2005.09.002

Immunostimulatory principles from Chlorella pyrenoidosa-Part 1: Isolation and biological assessment in vitro

J. A. Kralovec, K. L. Metera, J. R. Kumar, L. V. Watson, G. S. Girouard, Y. Guan, R. I. Carr, C. J. Barrow, H. S. Ewart

Medicine

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65 Citas (Scopus)

Resumen

Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin™), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin™ is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin™ is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.

Idioma original	English
Páginas (desde-hasta)	57-64
Número de páginas	8
Publicación	Phytomedicine
Volumen	14
N.º	1
DOI	https://doi.org/10.1016/j.phymed.2005.09.002
Estado	Published - ene. 10 2007

Nota bibliográfica

Funding Information:
This study was supported in part by the IRAP program of the National Research Council of Canada.

ASJC Scopus Subject Areas

Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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title = "Immunostimulatory principles from Chlorella pyrenoidosa-Part 1: Isolation and biological assessment in vitro",

abstract = "Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin{\texttrademark}), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin{\texttrademark} is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin{\texttrademark} is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.",

author = "Kralovec, {J. A.} and Metera, {K. L.} and Kumar, {J. R.} and Watson, {L. V.} and Girouard, {G. S.} and Y. Guan and Carr, {R. I.} and Barrow, {C. J.} and Ewart, {H. S.}",

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AU - Kralovec, J. A.

AU - Metera, K. L.

AU - Kumar, J. R.

AU - Watson, L. V.

AU - Girouard, G. S.

AU - Guan, Y.

AU - Carr, R. I.

AU - Barrow, C. J.

AU - Ewart, H. S.

N1 - Funding Information: This study was supported in part by the IRAP program of the National Research Council of Canada.

PY - 2007/1/10

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AB - Our proprietary preparation obtained by extraction of Chlorella pyrenoidosa cells, ONC-107 (Respondin™), was recently found to selectively boost antibody response to the influenza vaccine in a human clinical trial. Respondin™ is a potent stimulator of mouse B cell proliferation and an activator of macrophages. Bioactivity-guided resolution concluded that Respondin™ is composed of a mixture of immunostimulatory principles of different chemical nature. A combination of size exclusion, anion exchange and hydrophobic interaction chromatography revealed that the bulk of the immunostimulatory activity resides in polysaccharide/protein complexes with molecular masses larger than 100 kDa that are composed primarily of galactose, rhamnose and arabinose.

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Immunostimulatory principles from Chlorella pyrenoidosa-Part 1: Isolation and biological assessment in vitro

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

ODS de las Naciones Unidas

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