Increased DNA polymerase activity in Escherichia coli C after infection with F{cyrillic}X174

T. Y. Huh; C. W. Helleiner

doi:10.1016/0005-2787(68)90340-7

Increased DNA polymerase activity in Escherichia coli C after infection with F{cyrillic}X174

T. Y. Huh, C. W. Helleiner

Medicine

Medicine

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2 Citas (Scopus)

Resumen

The activity of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) in extracts of Escherichia coli C, synchronously infected with bacteriophage F{cyrillic}X174 increases by a factor of between 2 and 5, 20 min after the initiation of virus development. F{cyrillic}X174 irradiated with ultraviolet light did not elicit this response. The optimum Mg²⁺ and K⁺ concentrations of DNA polymerase in extracts from control E. coli and from those 20 min after infection differed considerably. Both DNA polymerases showed an apparent increase in activity when preheated at temperatures below 55°, but the enzyme from infected E. coli showed a greater increase. DNA polymerase from infected E. coli was purified approx. 1000-fold by means of fractionation with (NH₄)₂SO₄ and chromatography on DEAE-cellulose.

Idioma original	English
Páginas (desde-hasta)	107-113
Número de páginas	7
Publicación	BBA Section Nucleic Acids And Protein Synthesis
Volumen	155
N.º	1
DOI	https://doi.org/10.1016/0005-2787(68)90340-7
Estado	Published - ene. 29 1968

Nota bibliográfica

Funding Information:
The financial support of the Medical Research Council of Canada and the National Cancer Institute of Canada is gratefully acknowledged. The authors wish to express their appreciation of the assistance of D. A. WINTER in operating the Biogen.

ASJC Scopus Subject Areas

General Medicine

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title = "Increased DNA polymerase activity in Escherichia coli C after infection with F{cyrillic}X174",

abstract = "The activity of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) in extracts of Escherichia coli C, synchronously infected with bacteriophage F{cyrillic}X174 increases by a factor of between 2 and 5, 20 min after the initiation of virus development. F{cyrillic}X174 irradiated with ultraviolet light did not elicit this response. The optimum Mg2+ and K+ concentrations of DNA polymerase in extracts from control E. coli and from those 20 min after infection differed considerably. Both DNA polymerases showed an apparent increase in activity when preheated at temperatures below 55°, but the enzyme from infected E. coli showed a greater increase. DNA polymerase from infected E. coli was purified approx. 1000-fold by means of fractionation with (NH4)2SO4 and chromatography on DEAE-cellulose.",

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AU - Helleiner, C. W.

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N2 - The activity of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) in extracts of Escherichia coli C, synchronously infected with bacteriophage F{cyrillic}X174 increases by a factor of between 2 and 5, 20 min after the initiation of virus development. F{cyrillic}X174 irradiated with ultraviolet light did not elicit this response. The optimum Mg2+ and K+ concentrations of DNA polymerase in extracts from control E. coli and from those 20 min after infection differed considerably. Both DNA polymerases showed an apparent increase in activity when preheated at temperatures below 55°, but the enzyme from infected E. coli showed a greater increase. DNA polymerase from infected E. coli was purified approx. 1000-fold by means of fractionation with (NH4)2SO4 and chromatography on DEAE-cellulose.

AB - The activity of DNA polymerase (deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) in extracts of Escherichia coli C, synchronously infected with bacteriophage F{cyrillic}X174 increases by a factor of between 2 and 5, 20 min after the initiation of virus development. F{cyrillic}X174 irradiated with ultraviolet light did not elicit this response. The optimum Mg2+ and K+ concentrations of DNA polymerase in extracts from control E. coli and from those 20 min after infection differed considerably. Both DNA polymerases showed an apparent increase in activity when preheated at temperatures below 55°, but the enzyme from infected E. coli showed a greater increase. DNA polymerase from infected E. coli was purified approx. 1000-fold by means of fractionation with (NH4)2SO4 and chromatography on DEAE-cellulose.

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Increased DNA polymerase activity in Escherichia coli C after infection with F{cyrillic}X174

Resumen

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ASJC Scopus Subject Areas

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