Individual and interactive effects of temperature and light intensity on canola growth, physiological characteristics and methane emissions

Earlier studies have shown that plants produce methane (CH₄) under aerobic conditions, and that this emission is not microbial in nature. However, the precursors of aerobic CH₄ remain under debate, and the combined effects of environmental factors on plant-derived CH₄ requires further attention. The objective of this study was to determine the interactive effects of temperature and light intensity on CH₄ and other relevant plant parameters in canola (Brassica napus L.). Plants were grown under two temperature regimes (22/18 °C and 28/24 °C, 16 h light/8 h dark) and two light intensities (300 and 600 μmol photons m⁻² s⁻¹) for 21 days after one week of growth under 22/18 °C (16 h light/8 h dark). In this study, higher temperature had little effects on CH₄ emissions from plants, indicating the mitigating effects of higher light intensity. Higher light intensity, however, significantly decreased CH₄, which was inversely related to plant dry mass. Higher light intensity decreased stem height, leaf area ratio, chlorophyll, nitrogen balance index, leaf moisture, methionine (Met) and ethylene (C₂H₄), but increased specific leaf mass, photochemical quenching, flavonoids, epicuticular wax, lysine and tyrosine. The results revealed that increased CH₄ emissions from plants could be related to changes in plant physiological activities, which portrayed themselves in increased C₂H₄ evolution, and methylated amino acids, such as Met. We conclude that higher light intensity reduces Met and, in turn, CH₄ and C₂H₄ emissions, but lower light intensity enhances CH₄ formation through cleavage of methyl group of amino acids by reactive oxygen species, as previously suggested.