TY - JOUR
T1 - Indomethacin blocks the febrile response induced by interleukin-8 in rabbits
AU - Zampronio, A. R.
AU - Silva, C. A.A.
AU - Cunha, F. Q.
AU - Ferreira, S. H.
AU - Pela, I. R.
AU - Souza, G. E.P.
PY - 1995
Y1 - 1995
N2 - Interleukin (IL)-8 induces fever in rats by a mechanism independent of the release of cyclooxygenase products. The purpose of this study was to investigate whether a similar mechanism is responsible for the pyrogenic effect of IL-8 in rabbits. Intravenous (0.31-5.0 ng/kg) or intracerebroventricular (15.6-250 pg) injections of IL-8 induced a dose- dependent increase in body temperature. The correlations between the doses of recombinant human IL-8 and the fever index were 0.98 and 0.99 for the intravenous and intracerebroventricular injections, respectively. The pyrogenic activity of IL-8 was not due to contamination with lipopolysaccharide (LPS), inasmuch as the Limulus amoebocyte lysate test showed < 10 pg endotoxin/μg IL-8, and boiled IL-8 lost its pyrogenic activity. Indomethacin (2 and 5 mg/kg ip) abolished the febrile response induced by the intravenous injection of LPS (5.0 ng/kg), IL-1β (5 ng/kg), and IL-8 (5 ng/kg). Indomethacin also abolished the fever induced by the intracerebroventricular injection of IL-8 (62.5 pg) but only partially reduced the response induced by the injection of IL-1β (25 pg icv). These results show that, different from rats, indomethacin blocks the febrile response induced by the central or peripheral administration of IL-8 in rabbits.
AB - Interleukin (IL)-8 induces fever in rats by a mechanism independent of the release of cyclooxygenase products. The purpose of this study was to investigate whether a similar mechanism is responsible for the pyrogenic effect of IL-8 in rabbits. Intravenous (0.31-5.0 ng/kg) or intracerebroventricular (15.6-250 pg) injections of IL-8 induced a dose- dependent increase in body temperature. The correlations between the doses of recombinant human IL-8 and the fever index were 0.98 and 0.99 for the intravenous and intracerebroventricular injections, respectively. The pyrogenic activity of IL-8 was not due to contamination with lipopolysaccharide (LPS), inasmuch as the Limulus amoebocyte lysate test showed < 10 pg endotoxin/μg IL-8, and boiled IL-8 lost its pyrogenic activity. Indomethacin (2 and 5 mg/kg ip) abolished the febrile response induced by the intravenous injection of LPS (5.0 ng/kg), IL-1β (5 ng/kg), and IL-8 (5 ng/kg). Indomethacin also abolished the fever induced by the intracerebroventricular injection of IL-8 (62.5 pg) but only partially reduced the response induced by the injection of IL-1β (25 pg icv). These results show that, different from rats, indomethacin blocks the febrile response induced by the central or peripheral administration of IL-8 in rabbits.
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U2 - 10.1152/ajpregu.1995.269.6.r1469
DO - 10.1152/ajpregu.1995.269.6.r1469
M3 - Article
C2 - 8594951
AN - SCOPUS:0029585486
SN - 0363-6119
VL - 269
SP - R1469-R1474
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 6 38-6
ER -