Indomethacin blocks the febrile response induced by interleukin-8 in rabbits

A. R. Zampronio, C. A.A. Silva, F. Q. Cunha, S. H. Ferreira, I. R. Pela, G. E.P. Souza

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

25 Citas (Scopus)

Resumen

Interleukin (IL)-8 induces fever in rats by a mechanism independent of the release of cyclooxygenase products. The purpose of this study was to investigate whether a similar mechanism is responsible for the pyrogenic effect of IL-8 in rabbits. Intravenous (0.31-5.0 ng/kg) or intracerebroventricular (15.6-250 pg) injections of IL-8 induced a dose- dependent increase in body temperature. The correlations between the doses of recombinant human IL-8 and the fever index were 0.98 and 0.99 for the intravenous and intracerebroventricular injections, respectively. The pyrogenic activity of IL-8 was not due to contamination with lipopolysaccharide (LPS), inasmuch as the Limulus amoebocyte lysate test showed < 10 pg endotoxin/μg IL-8, and boiled IL-8 lost its pyrogenic activity. Indomethacin (2 and 5 mg/kg ip) abolished the febrile response induced by the intravenous injection of LPS (5.0 ng/kg), IL-1β (5 ng/kg), and IL-8 (5 ng/kg). Indomethacin also abolished the fever induced by the intracerebroventricular injection of IL-8 (62.5 pg) but only partially reduced the response induced by the injection of IL-1β (25 pg icv). These results show that, different from rats, indomethacin blocks the febrile response induced by the central or peripheral administration of IL-8 in rabbits.

Idioma originalEnglish
Páginas (desde-hasta)R1469-R1474
PublicaciónAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volumen269
N.º6 38-6
DOI
EstadoPublished - 1995
Publicado de forma externa

ASJC Scopus Subject Areas

  • Physiology
  • Physiology (medical)

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