Intracellular murine hepatitis virus-specific RNAs contain common sequences

Steve Cheley; Robert Anderson; Margaret J. Cupples; Edwin C.M. Lee Chan; Vincent L. Morris

doi:10.1016/0042-6822(81)90305-6

Intracellular murine hepatitis virus-specific RNAs contain common sequences

Steve Cheley, Robert Anderson, Margaret J. Cupples, Edwin C.M. Lee Chan, Vincent L. Morris

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28 Citas (Scopus)

Resumen

A major polyadenylated viral RNA of approximately 0.8 × 10⁶ daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded ³²P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10⁶ daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.

Idioma original	English
Páginas (desde-hasta)	596-604
Número de páginas	9
Publicación	Virology
Volumen	112
N.º	2
DOI	https://doi.org/10.1016/0042-6822(81)90305-6
Estado	Published - jul. 30 1981
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
We are grateful to H. Shida for advice and assistance with the cell-free translation system. We also thank R. Hartwick for his help with the photography. This work was supportedb y grants from the Medical Research Council of Canada, Grant MA-5941 to R. Anderson and Grant MA-7321 to V. L. Morris.

ASJC Scopus Subject Areas

Virology

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title = "Intracellular murine hepatitis virus-specific RNAs contain common sequences",

abstract = "A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.",

author = "Steve Cheley and Robert Anderson and Cupples, {Margaret J.} and {Lee Chan}, {Edwin C.M.} and Morris, {Vincent L.}",

note = "Funding Information: We are grateful to H. Shida for advice and assistance with the cell-free translation system. We also thank R. Hartwick for his help with the photography. This work was supportedb y grants from the Medical Research Council of Canada, Grant MA-5941 to R. Anderson and Grant MA-7321 to V. L. Morris.",

year = "1981",

month = jul,

day = "30",

doi = "10.1016/0042-6822(81)90305-6",

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T1 - Intracellular murine hepatitis virus-specific RNAs contain common sequences

AU - Cheley, Steve

AU - Anderson, Robert

AU - Cupples, Margaret J.

AU - Lee Chan, Edwin C.M.

AU - Morris, Vincent L.

N1 - Funding Information: We are grateful to H. Shida for advice and assistance with the cell-free translation system. We also thank R. Hartwick for his help with the photography. This work was supportedb y grants from the Medical Research Council of Canada, Grant MA-5941 to R. Anderson and Grant MA-7321 to V. L. Morris.

PY - 1981/7/30

Y1 - 1981/7/30

N2 - A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.

AB - A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.

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Intracellular murine hepatitis virus-specific RNAs contain common sequences

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

ODS de las Naciones Unidas

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