TY - JOUR
T1 - Isolation and characterization of tetranucleotide microsatellites from atlantic haddock (Melanogrammus aeglefinus)
AU - O'Reilly, Patrick T.
AU - McPherson, Arran A.
AU - Kenchington, Ellen
AU - Taggart, Chris
AU - Jones, Mathew W.
AU - Bentzen, Paul
PY - 2002/7
Y1 - 2002/7
N2 - Five tetranucleotide microsatellite loci developed from Atlantic haddock (Melanogrammus aeglefinus) are presented. Loci were isolated using a modified magnetic bead-hybridization selection procedure that enriched for tetranucleotide microsatellites. Loci were polymorphic (3-99 alleles per locus; mean, 33.2) and exhibited high levels of observed heterozygosity (0.07-0.99; mean, 0.73) in a sample of 70 haddock collected from the Scotian Shelf in the Northwest Atlantic Ocean. Primer sets for 5 tetranucleotide microsatellites, originally developed from cod (Gmo34), were also tested in Atlantic haddock; one pair yielded readily detectable product and was variable in the population assayed (29 alleles; heterozygosity, 0.96). These loci are suitable for kinship analyses in aquaculture-related applications, and are potentially useful for resolving population structure in the wild.
AB - Five tetranucleotide microsatellite loci developed from Atlantic haddock (Melanogrammus aeglefinus) are presented. Loci were isolated using a modified magnetic bead-hybridization selection procedure that enriched for tetranucleotide microsatellites. Loci were polymorphic (3-99 alleles per locus; mean, 33.2) and exhibited high levels of observed heterozygosity (0.07-0.99; mean, 0.73) in a sample of 70 haddock collected from the Scotian Shelf in the Northwest Atlantic Ocean. Primer sets for 5 tetranucleotide microsatellites, originally developed from cod (Gmo34), were also tested in Atlantic haddock; one pair yielded readily detectable product and was variable in the population assayed (29 alleles; heterozygosity, 0.96). These loci are suitable for kinship analyses in aquaculture-related applications, and are potentially useful for resolving population structure in the wild.
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U2 - 10.1007/s10126-002-0010-4
DO - 10.1007/s10126-002-0010-4
M3 - Article
AN - SCOPUS:0036658938
SN - 1436-2228
VL - 4
SP - 418
EP - 422
JO - Marine Biotechnology
JF - Marine Biotechnology
IS - 4
ER -