Isolation of nine novel tetranucleotide microsatellites in Atlantic herring (Clupea harengus)

A. A. Mcpherson; P. T. O'Reilly; T. L. Mcparland; M. W. Jones; P. Bentzen

doi:10.1046/j.1471-8278.2000.00012.x

Isolation of nine novel tetranucleotide microsatellites in Atlantic herring (Clupea harengus)

A. A. Mcpherson, P. T. O'Reilly, T. L. Mcparland, M. W. Jones, P. Bentzen

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Resumen

Nine tetranucleotide microsatellite loci developed for Atlantic herring are presented. Loci were isolated using a magnetic bead hybridization selection protocol that yielded an 80% enrichment for tetranucleotide microsatellites. Fifty Atlantic herring from each of a North-west and North-east Atlantic spawning group were screened at each locus. Loci were polymorphic (12-56 alleles per locus) and exhibited high levels of observed heterozygosity (0.66-0.98). These loci are therefore suitable for population studies and the analysis of mating.

Idioma original	English
Páginas (desde-hasta)	31-32
Número de páginas	2
Publicación	Molecular Ecology Notes
Volumen	1
N.º	1-2
DOI	https://doi.org/10.1046/j.1471-8278.2000.00012.x
Estado	Published - 2001
Publicado de forma externa	Sí

ASJC Scopus Subject Areas

Biochemistry
Ecology
General Biochemistry,Genetics and Molecular Biology

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AU - Mcpherson, A. A.

AU - O'Reilly, P. T.

AU - Mcparland, T. L.

AU - Jones, M. W.

AU - Bentzen, P.

PY - 2001

Y1 - 2001

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AB - Nine tetranucleotide microsatellite loci developed for Atlantic herring are presented. Loci were isolated using a magnetic bead hybridization selection protocol that yielded an 80% enrichment for tetranucleotide microsatellites. Fifty Atlantic herring from each of a North-west and North-east Atlantic spawning group were screened at each locus. Loci were polymorphic (12-56 alleles per locus) and exhibited high levels of observed heterozygosity (0.66-0.98). These loci are therefore suitable for population studies and the analysis of mating.

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Isolation of nine novel tetranucleotide microsatellites in Atlantic herring (Clupea harengus)

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ASJC Scopus Subject Areas

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