Kinetics of etomidate actions on GABAA receptors in the rat spinal dorsal horn neurons

Zhen Xiong Zhang; Hui Lü; Xian Ping Dong; Jin Liu; Tian Le Xu

doi:10.1016/S0006-8993(02)03274-2

Kinetics of etomidate actions on GABA_A receptors in the rat spinal dorsal horn neurons

Zhen Xiong Zhang, Hui Lü, Xian Ping Dong, Jin Liu, Tian Le Xu

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

20 Citas (Scopus)

Resumen

Electrophysiological properties of etomidate (ET)-induced current (I_ET) at different concentrations and effects of ET at clinically relevant concentrations (1-10 μM) on postsynaptic GABA_A receptor function were investigated using whole-cell patch-clamp technique in mechanically dissociated rat spinal dorsal horn neurons. The results showed that ET actions were concentration-dependent: low concentrations (10 μM) of ET potentiated GABA-activated current (I_GABA), slowed activation, desensitization and deactivation of GABA_A receptors; moderate concentrations (10-1000 μM) of ET directly activated and desensitized GABA_A receptors; high concentrations (>1000 μM) of ET produced an inhibitory effect on I_ET. In addition, ET prolonged the duration of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) in the mechanically dissociated rat dorsal horn neurons. These results suggest that general anesthetics-induced changes at spinal level could significantly contribute to analgesia and general anesthesia.

Idioma original	English
Páginas (desde-hasta)	93-100
Número de páginas	8
Publicación	Brain Research
Volumen	953
N.º	1-2
DOI	https://doi.org/10.1016/S0006-8993(02)03274-2
Estado	Published - nov. 20 2002
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
We thank Drs. Lin Chen and Lin Xu for their excellent editorial help. This work was supported by the Grant for Outstanding Young Researchers from the Ministry of Education of China, the National Natural Science Foundation of China (nos. 30125015, 39970200, 30170247) and the National Basic Research Program of China (G1999054000) to T.-L. Xu.

ASJC Scopus Subject Areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(02)03274-2

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title = "Kinetics of etomidate actions on GABAA receptors in the rat spinal dorsal horn neurons",

abstract = "Electrophysiological properties of etomidate (ET)-induced current (IET) at different concentrations and effects of ET at clinically relevant concentrations (1-10 μM) on postsynaptic GABAA receptor function were investigated using whole-cell patch-clamp technique in mechanically dissociated rat spinal dorsal horn neurons. The results showed that ET actions were concentration-dependent: low concentrations (10 μM) of ET potentiated GABA-activated current (IGABA), slowed activation, desensitization and deactivation of GABAA receptors; moderate concentrations (10-1000 μM) of ET directly activated and desensitized GABAA receptors; high concentrations (>1000 μM) of ET produced an inhibitory effect on IET. In addition, ET prolonged the duration of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) in the mechanically dissociated rat dorsal horn neurons. These results suggest that general anesthetics-induced changes at spinal level could significantly contribute to analgesia and general anesthesia.",

author = "Zhang, {Zhen Xiong} and Hui L{\"u} and Dong, {Xian Ping} and Jin Liu and Xu, {Tian Le}",

note = "Funding Information: We thank Drs. Lin Chen and Lin Xu for their excellent editorial help. This work was supported by the Grant for Outstanding Young Researchers from the Ministry of Education of China, the National Natural Science Foundation of China (nos. 30125015, 39970200, 30170247) and the National Basic Research Program of China (G1999054000) to T.-L. Xu.",

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AU - Lü, Hui

AU - Dong, Xian Ping

AU - Liu, Jin

AU - Xu, Tian Le

N1 - Funding Information: We thank Drs. Lin Chen and Lin Xu for their excellent editorial help. This work was supported by the Grant for Outstanding Young Researchers from the Ministry of Education of China, the National Natural Science Foundation of China (nos. 30125015, 39970200, 30170247) and the National Basic Research Program of China (G1999054000) to T.-L. Xu.

PY - 2002/11/20

Y1 - 2002/11/20

N2 - Electrophysiological properties of etomidate (ET)-induced current (IET) at different concentrations and effects of ET at clinically relevant concentrations (1-10 μM) on postsynaptic GABAA receptor function were investigated using whole-cell patch-clamp technique in mechanically dissociated rat spinal dorsal horn neurons. The results showed that ET actions were concentration-dependent: low concentrations (10 μM) of ET potentiated GABA-activated current (IGABA), slowed activation, desensitization and deactivation of GABAA receptors; moderate concentrations (10-1000 μM) of ET directly activated and desensitized GABAA receptors; high concentrations (>1000 μM) of ET produced an inhibitory effect on IET. In addition, ET prolonged the duration of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) in the mechanically dissociated rat dorsal horn neurons. These results suggest that general anesthetics-induced changes at spinal level could significantly contribute to analgesia and general anesthesia.

AB - Electrophysiological properties of etomidate (ET)-induced current (IET) at different concentrations and effects of ET at clinically relevant concentrations (1-10 μM) on postsynaptic GABAA receptor function were investigated using whole-cell patch-clamp technique in mechanically dissociated rat spinal dorsal horn neurons. The results showed that ET actions were concentration-dependent: low concentrations (10 μM) of ET potentiated GABA-activated current (IGABA), slowed activation, desensitization and deactivation of GABAA receptors; moderate concentrations (10-1000 μM) of ET directly activated and desensitized GABAA receptors; high concentrations (>1000 μM) of ET produced an inhibitory effect on IET. In addition, ET prolonged the duration of GABAergic miniature inhibitory postsynaptic currents (mIPSCs) in the mechanically dissociated rat dorsal horn neurons. These results suggest that general anesthetics-induced changes at spinal level could significantly contribute to analgesia and general anesthesia.

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