Long-duration freewheel running and submandibular lymphocyte response to forced exercise in older mice

J. Boudreau, L. Hoffman-Goetz

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2 Citas (Scopus)

Resumen

Submandibular lymph nodes (SLN) are crucial for immune surveillance of the anterior ocular chamber and upper respiratory tract; little is known about how training and exercise affect SLN lymphocytes. The intent of this study was to describe the impact of long term freewheel running followed by acute strenuous exercise on SLN lymphocytes in mice. Female C57BL/6 mice were assigned to running wheels or remained sedentary for 8 months, and further randomized to treadmill exercise and sacrifice immediately, treadmill exercise and sacrifice 24 h after exercise cessation, or no treadmill exposure. SLN lymphocytes were isolated and analyzed for CD3, CD4, CD8, and CD19 cell surface markers, phosphatidylserine externalization as a marker of apoptosis, and intracellular glutathione as a marker of oxidative stress. Compared with running wheel mice, older sedentary mice had a lower percent of T cells and higher percent of B cells (p < 0.05). Although intracellular glutathione did not differ between groups, running mice had a lower percent of Annexin V+ SLN lymphocytes 24 h after treadmill exercise. Further research will be needed to determine if voluntary exercise translates into improved anterior ocular and upper respiratory tract health.

Idioma originalEnglish
Páginas (desde-hasta)565-572
Número de páginas8
PublicaciónCanadian Journal of Physiology and Pharmacology
Volumen84
N.º5
DOI
EstadoPublished - 2006
Publicado de forma externa

ASJC Scopus Subject Areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

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