Mass spectrometry analysis of intact proteins recovered from silver stained gels

Victor J. Nesatyy; Alan Doucette; Neil W. Ross

Mass spectrometry analysis of intact proteins recovered from silver stained gels

Victor J. Nesatyy, Alan Doucette, Neil W. Ross

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Resumen

The recovery of intact proteins from fixed silver stained gels on a wide molecular weight range and MALDI-MS in the presence of detergent was analyzed. Experiments were performed on protein standards of a wide molecular weight range. Electrophoresis was performed on freshly cast 0.75 mm thick, 12% polyacrylamide gels, using the Mini-Prorean system according to the method of Laemmli. Results on the recovery and MS analysis of intact gel-separated silver stained proteins were presented. The preliminary results on the MS analysis of silver stained proteins recovered from SDS gels were shown on the example of BSA.

Idioma original	English
Páginas	155-156
Número de páginas	2
Estado	Published - 2002
Publicado de forma externa	Sí
Evento	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duración: jun. 2 2002 → jun. 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
País/Territorio	United States
Ciudad	Orlando, FL
Período	6/2/02 → 6/6/02

ASJC Scopus Subject Areas

Spectroscopy

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title = "Mass spectrometry analysis of intact proteins recovered from silver stained gels",

abstract = "The recovery of intact proteins from fixed silver stained gels on a wide molecular weight range and MALDI-MS in the presence of detergent was analyzed. Experiments were performed on protein standards of a wide molecular weight range. Electrophoresis was performed on freshly cast 0.75 mm thick, 12% polyacrylamide gels, using the Mini-Prorean system according to the method of Laemmli. Results on the recovery and MS analysis of intact gel-separated silver stained proteins were presented. The preliminary results on the MS analysis of silver stained proteins recovered from SDS gels were shown on the example of BSA.",

author = "Nesatyy, {Victor J.} and Alan Doucette and Ross, {Neil W.}",

year = "2002",

language = "English",

pages = "155--156",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - Mass spectrometry analysis of intact proteins recovered from silver stained gels

AU - Nesatyy, Victor J.

AU - Doucette, Alan

AU - Ross, Neil W.

PY - 2002

Y1 - 2002

N2 - The recovery of intact proteins from fixed silver stained gels on a wide molecular weight range and MALDI-MS in the presence of detergent was analyzed. Experiments were performed on protein standards of a wide molecular weight range. Electrophoresis was performed on freshly cast 0.75 mm thick, 12% polyacrylamide gels, using the Mini-Prorean system according to the method of Laemmli. Results on the recovery and MS analysis of intact gel-separated silver stained proteins were presented. The preliminary results on the MS analysis of silver stained proteins recovered from SDS gels were shown on the example of BSA.

AB - The recovery of intact proteins from fixed silver stained gels on a wide molecular weight range and MALDI-MS in the presence of detergent was analyzed. Experiments were performed on protein standards of a wide molecular weight range. Electrophoresis was performed on freshly cast 0.75 mm thick, 12% polyacrylamide gels, using the Mini-Prorean system according to the method of Laemmli. Results on the recovery and MS analysis of intact gel-separated silver stained proteins were presented. The preliminary results on the MS analysis of silver stained proteins recovered from SDS gels were shown on the example of BSA.

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UR - http://www.scopus.com/inward/citedby.url?scp=2442667967&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442667967

SP - 155

EP - 156

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Mass spectrometry analysis of intact proteins recovered from silver stained gels

Resumen

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ASJC Scopus Subject Areas

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