Methods for monitoring Ca2+ and ion channels in the lysosome

Xi Zoë Zhong, Yiming Yang, Xue Sun, Xian Ping Dong

Producción científica: Contribución a una revistaArtículo de revisiónrevisión exhaustiva

25 Citas (Scopus)

Resumen

Lysosomes and lysosome-related organelles are emerging as intracellular Ca2+ stores and play important roles in a variety of membrane trafficking processes, including endocytosis, exocytosis, phagocytosis and autophagy. Impairment of lysosomal Ca2+ homeostasis and membrane trafficking has been implicated in many human diseases such as lysosomal storage diseases (LSDs), neurodegeneration, myopathy and cancer. Lysosomal membrane proteins, in particular ion channels, are crucial for lysosomal Ca2+ signaling. Compared with ion channels in the plasma membrane, lysosomal ion channels and their roles in lysosomal Ca2+ signaling are less understood, largely due to their intracellular localization and the lack of feasible functional assays directly applied to the native environment. Recent advances in biomedical methodology have made it possible to directly investigate ion channels in the lysosomal membrane. In this review, we provide a summary of the newly developed methods for monitoring lysosomal Ca2+ and ion channels, as well as the recent discovery of lysosomal ion channels and their significances in intracellular Ca2+ signaling. These new techniques will expand our research scope and our understanding of the nature of lysosomes and lysosome-related diseases.

Idioma originalEnglish
Páginas (desde-hasta)20-28
Número de páginas9
PublicaciónCell Calcium
Volumen64
DOI
EstadoPublished - jun. 2017

Nota bibliográfica

Funding Information:
We apologize to colleagues whose works are not cited due to space limitations. The work in the Dong laboratory is funded by CIHR grant (MOP-119349) and CFI Leaders Opportunity Fund-Funding for research infrastructure (29291).

Publisher Copyright:
© 2016

ASJC Scopus Subject Areas

  • Physiology
  • Molecular Biology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article
  • Review
  • Research Support, Non-U.S. Gov't

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