Microscopic and spectroscopic analysis of chitosan-DNA conjugates

D. Agudelo, L. Kreplak, H. A. Tajmir-Riahi

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

24 Citas (Scopus)

Resumen

Conjugations of DNA with chitosans 15 kD (ch-15), 100 kD (ch-100) and 200 kD (ch-200) were investigated in aqueous solution at pH 5.5-6.5. Multiple spectroscopic methods and atomic force microscopy (AFM) were used to locate the chitosan binding sites and the effect of polymer conjugation on DNA compaction and particle formation. Structural analysis showed that chitosan-DNA conjugation is mainly via electrostatic interactions through polymer cationic charged NH2 and negatively charged backbone phosphate groups. As polymer size increases major DNA compaction and particle formation occurs. At high chitosan concentration major DNA structural changes observed indicating a partial B to A-DNA conformational transition.

Idioma originalEnglish
Páginas (desde-hasta)207-213
Número de páginas7
PublicaciónCarbohydrate Polymers
Volumen137
DOI
EstadoPublished - feb. 10 2016

Nota bibliográfica

Funding Information:
This work is supported by grant from Natural Sciences and Engineering Research Council of Canada (NSERC) .

Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.

ASJC Scopus Subject Areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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