NADPH quinone oxidoreductase 1 mediates breast cancer cell resistance to thymoquinone-induced apoptosis

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Resumen

Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1.

Idioma originalEnglish
Páginas (desde-hasta)421-426
Número de páginas6
PublicaciónBiochemical and Biophysical Research Communications
Volumen426
N.º3
DOI
EstadoPublished - sep. 28 2012

Nota bibliográfica

Funding Information:
This work was supported by the Canadian Breast Cancer Foundation-Atlantic Region Endowed Chair awarded to D. Hoskin. K. Sutton was the recipient of a Natural Sciences and Engineering Research Council Postgraduate Studentship and a Trainee Award from the Cancer Research Training Program with funding from the Canadian Breast Cancer Foundation-Atlantic Region.

ASJC Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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