NADPH quinone oxidoreductase 1 mediates breast cancer cell resistance to thymoquinone-induced apoptosis

Kimberly M. Sutton; Carolyn D. Doucette; David W. Hoskin

doi:10.1016/j.bbrc.2012.08.111

NADPH quinone oxidoreductase 1 mediates breast cancer cell resistance to thymoquinone-induced apoptosis

Kimberly M. Sutton, Carolyn D. Doucette, David W. Hoskin

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

26 Citas (Scopus)

Resumen

Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1.

Idioma original	English
Páginas (desde-hasta)	421-426
Número de páginas	6
Publicación	Biochemical and Biophysical Research Communications
Volumen	426
N.º	3
DOI	https://doi.org/10.1016/j.bbrc.2012.08.111
Estado	Published - sep. 28 2012

Nota bibliográfica

Funding Information:
This work was supported by the Canadian Breast Cancer Foundation-Atlantic Region Endowed Chair awarded to D. Hoskin. K. Sutton was the recipient of a Natural Sciences and Engineering Research Council Postgraduate Studentship and a Trainee Award from the Cancer Research Training Program with funding from the Canadian Breast Cancer Foundation-Atlantic Region.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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title = "NADPH quinone oxidoreductase 1 mediates breast cancer cell resistance to thymoquinone-induced apoptosis",

abstract = "Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1.",

author = "Sutton, {Kimberly M.} and Doucette, {Carolyn D.} and Hoskin, {David W.}",

note = "Funding Information: This work was supported by the Canadian Breast Cancer Foundation-Atlantic Region Endowed Chair awarded to D. Hoskin. K. Sutton was the recipient of a Natural Sciences and Engineering Research Council Postgraduate Studentship and a Trainee Award from the Cancer Research Training Program with funding from the Canadian Breast Cancer Foundation-Atlantic Region.",

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AU - Doucette, Carolyn D.

AU - Hoskin, David W.

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PY - 2012/9/28

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N2 - Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1.

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NADPH quinone oxidoreductase 1 mediates breast cancer cell resistance to thymoquinone-induced apoptosis

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