Resumen
Thymoquinone (TQ), a bioactive component of black caraway seed (Nigella sativa) oil, is reported to have antineoplastic properties. In this study we investigated the effect of TQ on a panel of human breast cancer cell lines. Cell viability assays showed that TQ killed T-47D, MDA-MB-231, and MDA-MB-468 cells via p53-independent induction of apoptosis; however, MCF-7 cells were refractory to the cytotoxic action of TQ. Western Blot analysis showed that MCF-7 cells expressed high levels of cytoprotective NADPH quinone oxidoreductase 1 (NQO1), which was responsible for TQ-resistance since inhibition of NQO1 with dicoumarol rendered MCF-7 cells TQ-sensitive. These findings may be clinically important when considering TQ as a possible adjunct treatment for breast cancer since a high percentage of breast tumors express NQO1.
Idioma original | English |
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Páginas (desde-hasta) | 421-426 |
Número de páginas | 6 |
Publicación | Biochemical and Biophysical Research Communications |
Volumen | 426 |
N.º | 3 |
DOI | |
Estado | Published - sep. 28 2012 |
Nota bibliográfica
Funding Information:This work was supported by the Canadian Breast Cancer Foundation-Atlantic Region Endowed Chair awarded to D. Hoskin. K. Sutton was the recipient of a Natural Sciences and Engineering Research Council Postgraduate Studentship and a Trainee Award from the Cancer Research Training Program with funding from the Canadian Breast Cancer Foundation-Atlantic Region.
ASJC Scopus Subject Areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't