Noise analysis and single-channel observations of 4 pS chloride channels in human airway epithelia

M. Duszyk; A. S. French; S. F. Man

doi:10.1016/S0006-3495(92)81861-1

Noise analysis and single-channel observations of 4 pS chloride channels in human airway epithelia

M. Duszyk, A. S. French, S. F. Man

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Resumen

Apical membranes of human airway epithelial cells have significant chloride permeability, which is reduced in cystic fibrosis (CF), causing abnormal electrochemistry and impaired mucociliary clearance. At least four types of chloride channels have been identified in these cells, but their relative roles in total permeability and CF are unclear. Noise analysis was used to measure the conductance of chloride channels in human nasal epithelial cells. The data indicate that channels with a mean conductance of 4.5 pS carry most of the chloride current, and that the mean number of such channels per cell is approximately 4,000. Chloride channels in this conductance range were also seen in single-channel recordings.

Idioma original	English
Páginas (desde-hasta)	583-587
Número de páginas	5
Publicación	Biophysical Journal
Volumen	61
N.º	2
DOI	https://doi.org/10.1016/S0006-3495(92)81861-1
Estado	Published - 1992
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Supported by the Canadian Medical Research Council and the Canadian Cystic Fibrosis Foundation. Note added in proof: Dalemans et al. have found that normal and AF508 CFTR gene expression produce linear chloride channels of 4.9 and 4.3 pS, respectively. The open probability of the normal channels was - 0.3, as reported here for excised patches.

ASJC Scopus Subject Areas

Biophysics

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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title = "Noise analysis and single-channel observations of 4 pS chloride channels in human airway epithelia",

abstract = "Apical membranes of human airway epithelial cells have significant chloride permeability, which is reduced in cystic fibrosis (CF), causing abnormal electrochemistry and impaired mucociliary clearance. At least four types of chloride channels have been identified in these cells, but their relative roles in total permeability and CF are unclear. Noise analysis was used to measure the conductance of chloride channels in human nasal epithelial cells. The data indicate that channels with a mean conductance of 4.5 pS carry most of the chloride current, and that the mean number of such channels per cell is approximately 4,000. Chloride channels in this conductance range were also seen in single-channel recordings.",

author = "M. Duszyk and French, {A. S.} and Man, {S. F.}",

note = "Funding Information: Supported by the Canadian Medical Research Council and the Canadian Cystic Fibrosis Foundation. Note added in proof: Dalemans et al. have found that normal and AF508 CFTR gene expression produce linear chloride channels of 4.9 and 4.3 pS, respectively. The open probability of the normal channels was - 0.3, as reported here for excised patches.",

year = "1992",

doi = "10.1016/S0006-3495(92)81861-1",

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T1 - Noise analysis and single-channel observations of 4 pS chloride channels in human airway epithelia

AU - Duszyk, M.

AU - French, A. S.

AU - Man, S. F.

N1 - Funding Information: Supported by the Canadian Medical Research Council and the Canadian Cystic Fibrosis Foundation. Note added in proof: Dalemans et al. have found that normal and AF508 CFTR gene expression produce linear chloride channels of 4.9 and 4.3 pS, respectively. The open probability of the normal channels was - 0.3, as reported here for excised patches.

PY - 1992

Y1 - 1992

N2 - Apical membranes of human airway epithelial cells have significant chloride permeability, which is reduced in cystic fibrosis (CF), causing abnormal electrochemistry and impaired mucociliary clearance. At least four types of chloride channels have been identified in these cells, but their relative roles in total permeability and CF are unclear. Noise analysis was used to measure the conductance of chloride channels in human nasal epithelial cells. The data indicate that channels with a mean conductance of 4.5 pS carry most of the chloride current, and that the mean number of such channels per cell is approximately 4,000. Chloride channels in this conductance range were also seen in single-channel recordings.

AB - Apical membranes of human airway epithelial cells have significant chloride permeability, which is reduced in cystic fibrosis (CF), causing abnormal electrochemistry and impaired mucociliary clearance. At least four types of chloride channels have been identified in these cells, but their relative roles in total permeability and CF are unclear. Noise analysis was used to measure the conductance of chloride channels in human nasal epithelial cells. The data indicate that channels with a mean conductance of 4.5 pS carry most of the chloride current, and that the mean number of such channels per cell is approximately 4,000. Chloride channels in this conductance range were also seen in single-channel recordings.

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Noise analysis and single-channel observations of 4 pS chloride channels in human airway epithelia

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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