TY - JOUR
T1 - Nonreplicating viral vectors as potential vaccines
T2 - Recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins
AU - Taylor, Jill
AU - Weinberg, Randall
AU - Tartaglia, James
AU - Richardson, Christopher
AU - Alkhatib, Ghalib
AU - Briedis, Dalius
AU - Appel, Max
AU - Norton, Elizabeth
AU - Paoletti, Enzo
PY - 1992/3
Y1 - 1992/3
N2 - The development of canarypox virus (CPV) recombinants expressing the hemagglutinin (HA) and fusion (F) glycoproteins of measles virus (MV) is described. Inoculation of the CPV-MV recombinants into avian or nonavian tissue culture substrates led to the expression of authentic MVF and MVHA as determined by radioimmunoprecipitation and surface immunofluorescence. In contrast to avian-derived tissue culture, no productive replication of the CPV recombinant was evident in tissue culture cells derived from nonavian origin. On inoculation of dogs, a species restricted for avipoxvirus replication, the recombinants elicited a protective immune response against a lethal canine distemper virus (CDV) challenge. The level of MV neutralizing antibodies and the level of protection induced against CDV challenge achieved by the host-restricted CPV vector were equivalent to that obtained by vaccinia virus vectors expressing the same MV antigens.
AB - The development of canarypox virus (CPV) recombinants expressing the hemagglutinin (HA) and fusion (F) glycoproteins of measles virus (MV) is described. Inoculation of the CPV-MV recombinants into avian or nonavian tissue culture substrates led to the expression of authentic MVF and MVHA as determined by radioimmunoprecipitation and surface immunofluorescence. In contrast to avian-derived tissue culture, no productive replication of the CPV recombinant was evident in tissue culture cells derived from nonavian origin. On inoculation of dogs, a species restricted for avipoxvirus replication, the recombinants elicited a protective immune response against a lethal canine distemper virus (CDV) challenge. The level of MV neutralizing antibodies and the level of protection induced against CDV challenge achieved by the host-restricted CPV vector were equivalent to that obtained by vaccinia virus vectors expressing the same MV antigens.
UR - http://www.scopus.com/inward/record.url?scp=0026528306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026528306&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(92)90321-F
DO - 10.1016/0042-6822(92)90321-F
M3 - Article
C2 - 1736535
AN - SCOPUS:0026528306
SN - 0042-6822
VL - 187
SP - 321
EP - 328
JO - Virology
JF - Virology
IS - 1
ER -
