PHARMAKOLOGISCHE BEEINFLUSSBARKEIT DES INTESTINALEN REPERFUSIONSSCHADENS IM TIEREXPERIMENT

We evaluated experimentally (80 Lewis-rats) possible pharmacological strategies in the treatment of intestinal reperfusion injury in hypo- and normothermia. We used a specific perfusion solution containing PGI ₂ or radical scavengers (superoxide dismutase, oxypurinol, tocopherol, ascorbate). Decreased malondialdehyde (MDA) plasma release after reperfusion proved the antioxidative efficiency of the administered radical scavengers (normothermia - control group: MDA increase after 15 min of reperfusion to 160 ± 30% compared to level at the end of ischemia, oxypurinol: 110 ± 23%, tocopherol: 112 ± 12%, ascorbate: 104 ± 20%; p < 0,05). The ATP/ADP-ratio of the therapy groups was stable in contrast to the control group. Alkaline phosphatase release was significantly diminished under radical scavenger administration (normothermia/15 min reperfusion - control group: 7,7 ± 0,9 μmol/ls, oxypurinol: 4,4 ± 0,4 μmol/ls, tocopherol: 3,5 ± 0,1 μmol/ls, ascorbate: 5,9 ± 0,3 μmol/ls; p < 0,05). Histologically we observed a mucosa protective effect particularly in the ascorbate group. Other pharmacological strategies are discussed.