Previously undescribed thyroid-specific miRNA sequences in papillary thyroid carcinoma

Mateus Camargo Barros-Filho; Michelle Pewarchuk; Brenda de Carvalho Minatel; Adam P. Sage; Erin A. Marshall; Victor D. Martinez; Leigha D. Rock; Gavin MacAulay; Luiz Paulo Kowalski; Silvia Regina Rogatto; Cathie Garnis; Wan L. Lam

doi:10.1038/s10038-019-0583-7

Previously undescribed thyroid-specific miRNA sequences in papillary thyroid carcinoma

Mateus Camargo Barros-Filho, Michelle Pewarchuk, Brenda de Carvalho Minatel, Adam P. Sage, Erin A. Marshall, Victor D. Martinez, Leigha D. Rock, Gavin MacAulay, Luiz Paulo Kowalski, Silvia Regina Rogatto, Cathie Garnis, Wan L. Lam

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8 Citas (Scopus)

Resumen

Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, wherein diagnostic limitations and lack of accurate prognostic factors are important clinical challenges. In this study, we report the discovery of 234 novel miRNAs in non-neoplastic thyroid and PTC samples, obtained from publicly available small RNA sequencing datasets (TCGA and GEO). These sequences were observed to display similar molecular features compared to currently annotated miRNAs. These potentially novel miRNAs presented tissue-specificity and largely decreased expression in PTC compared to non-neoplastic samples. We showed that the disrupted novel miRNAs have diagnostic and prognostic potential, and were associated with BRAF mutation, a frequent alteration related to more aggressive PTC. In conclusion, our results expand the miRNA repertoire in thyroid tissues and highlight the potential biological role and clinical utility of previously unannotated miRNAs.

Idioma original	English
Páginas (desde-hasta)	505-508
Número de páginas	4
Publicación	Journal of Human Genetics
Volumen	64
N.º	5
DOI	https://doi.org/10.1038/s10038-019-0583-7
Estado	Published - may. 1 2019
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Acknowledgements This work was supported by grants from the Canadian Institutes for Health Research (CIHR FDN-143345) and the São Paulo Research Foundation (FAPESP 2015/20748-5). MCBF are supported by scholarships from FAPESP (2015/17707-5 and 2018/ 06138-8). BCM, APS, EAM and VDM are supported by scholarships from the University of British Columbia. APS, EAM and LDR are also supported by scholarships from CIHR. EAM is a Vanier Canada Graduate Scholar.

Publisher Copyright:
© 2019, The Author(s), under exclusive licence to The Japan Society of Human Genetics.

ASJC Scopus Subject Areas

Genetics
Genetics(clinical)

PubMed: MeSH publication types

Journal Article

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title = "Previously undescribed thyroid-specific miRNA sequences in papillary thyroid carcinoma",

abstract = "Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, wherein diagnostic limitations and lack of accurate prognostic factors are important clinical challenges. In this study, we report the discovery of 234 novel miRNAs in non-neoplastic thyroid and PTC samples, obtained from publicly available small RNA sequencing datasets (TCGA and GEO). These sequences were observed to display similar molecular features compared to currently annotated miRNAs. These potentially novel miRNAs presented tissue-specificity and largely decreased expression in PTC compared to non-neoplastic samples. We showed that the disrupted novel miRNAs have diagnostic and prognostic potential, and were associated with BRAF mutation, a frequent alteration related to more aggressive PTC. In conclusion, our results expand the miRNA repertoire in thyroid tissues and highlight the potential biological role and clinical utility of previously unannotated miRNAs.",

author = "Barros-Filho, {Mateus Camargo} and Michelle Pewarchuk and Minatel, {Brenda de Carvalho} and Sage, {Adam P.} and Marshall, {Erin A.} and Martinez, {Victor D.} and Rock, {Leigha D.} and Gavin MacAulay and Kowalski, {Luiz Paulo} and Rogatto, {Silvia Regina} and Cathie Garnis and Lam, {Wan L.}",

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AU - Barros-Filho, Mateus Camargo

AU - Pewarchuk, Michelle

AU - Minatel, Brenda de Carvalho

AU - Sage, Adam P.

AU - Marshall, Erin A.

AU - Martinez, Victor D.

AU - Rock, Leigha D.

AU - MacAulay, Gavin

AU - Kowalski, Luiz Paulo

AU - Rogatto, Silvia Regina

AU - Garnis, Cathie

AU - Lam, Wan L.

N1 - Funding Information: Acknowledgements This work was supported by grants from the Canadian Institutes for Health Research (CIHR FDN-143345) and the São Paulo Research Foundation (FAPESP 2015/20748-5). MCBF are supported by scholarships from FAPESP (2015/17707-5 and 2018/ 06138-8). BCM, APS, EAM and VDM are supported by scholarships from the University of British Columbia. APS, EAM and LDR are also supported by scholarships from CIHR. EAM is a Vanier Canada Graduate Scholar. Publisher Copyright: © 2019, The Author(s), under exclusive licence to The Japan Society of Human Genetics.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, wherein diagnostic limitations and lack of accurate prognostic factors are important clinical challenges. In this study, we report the discovery of 234 novel miRNAs in non-neoplastic thyroid and PTC samples, obtained from publicly available small RNA sequencing datasets (TCGA and GEO). These sequences were observed to display similar molecular features compared to currently annotated miRNAs. These potentially novel miRNAs presented tissue-specificity and largely decreased expression in PTC compared to non-neoplastic samples. We showed that the disrupted novel miRNAs have diagnostic and prognostic potential, and were associated with BRAF mutation, a frequent alteration related to more aggressive PTC. In conclusion, our results expand the miRNA repertoire in thyroid tissues and highlight the potential biological role and clinical utility of previously unannotated miRNAs.

AB - Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, wherein diagnostic limitations and lack of accurate prognostic factors are important clinical challenges. In this study, we report the discovery of 234 novel miRNAs in non-neoplastic thyroid and PTC samples, obtained from publicly available small RNA sequencing datasets (TCGA and GEO). These sequences were observed to display similar molecular features compared to currently annotated miRNAs. These potentially novel miRNAs presented tissue-specificity and largely decreased expression in PTC compared to non-neoplastic samples. We showed that the disrupted novel miRNAs have diagnostic and prognostic potential, and were associated with BRAF mutation, a frequent alteration related to more aggressive PTC. In conclusion, our results expand the miRNA repertoire in thyroid tissues and highlight the potential biological role and clinical utility of previously unannotated miRNAs.

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Previously undescribed thyroid-specific miRNA sequences in papillary thyroid carcinoma

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ASJC Scopus Subject Areas

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