Primary sequence and post-transcriptional modification pattern of an unusual mitochondrial tRNAMet from Tetrahymena pyriformis

Murray N. Schnare; Spencer J. Greenwood; Michael W. Gray

doi:10.1016/0014-5793(95)00179-D

Primary sequence and post-transcriptional modification pattern of an unusual mitochondrial tRNA^Met from Tetrahymena pyriformis

Murray N. Schnare, Spencer J. Greenwood, Michael W. Gray

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

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Resumen

In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA^Met gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be unusually small and have an atypical dihydrouridine stem-loop domain. We report here the complete nucleotide sequence and post-transcriptional modification pattern of this tRNA^Met, confirming its predicted primary structure and supporting the view that this structurally aberrant species functions in translation in T. pyriformis mitochondria.

Idioma original	English
Páginas (desde-hasta)	24-28
Número de páginas	5
Publicación	FEBS Letters
Volumen	362
N.º	1
DOI	https://doi.org/10.1016/0014-5793(95)00179-D
Estado	Published - mar. 27 1995
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
T. pyriform& strain used in this study, and T.Y.K. Heinonen for advice on isolation of mitochondria. This work was supported by an operating grant (MT-4124) from the Medical Research Council of Canada (M.W.G.), a Walter C. Sumner Memorial Fellowship (S.J.G.), and a Fellowship (M.W.G.) from the Canadian Institute for Advanced Research.

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

PubMed: MeSH publication types

Journal Article
Research Support, Non-U.S. Gov't

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10.1016/0014-5793(95)00179-D

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abstract = "In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNAMet gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be unusually small and have an atypical dihydrouridine stem-loop domain. We report here the complete nucleotide sequence and post-transcriptional modification pattern of this tRNAMet, confirming its predicted primary structure and supporting the view that this structurally aberrant species functions in translation in T. pyriformis mitochondria.",

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