Resumen
Costimulation by members of the B7 family of molecules is critical for the activation of naive CD4+ T cells. While prolonged TCR signaling is necessary for T cell activation, the duration of costimulatory signals required has not been established. In this study, murine bone marrow-derived dendritic cells (DC) and naïve CD4+ T cells were used to determine the temporal costimulatory requirements for naive T cell activation. By blocking CD80/CD86 costimulation at various time points during DC-T cell interaction and using the CFSE technique to assess the dynamics of T cell proliferation, we found that prolonged costimulation was required for naive T cells to enter and progress through the cell cycle over a wide range of peptide concentrations. Prolonged costimulation was also important for IL-2 production and CD25/CD69 expression by naive T cells. Video microscopy demonstrated that DC and naive T cells formed stable conjugates that persisted for more than 6 h. Thus, persistent CD80/CD86 signaling during prolonged interactions with DC allows naive T cells to enter the cell cycle and programs the daughter cells to undergo subsequent divisions.
Idioma original | English |
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Páginas (desde-hasta) | 135-143 |
Número de páginas | 9 |
Publicación | Immunology Letters |
Volumen | 106 |
N.º | 2 |
DOI | |
Estado | Published - ago. 15 2006 |
Nota bibliográfica
Funding Information:Supported by grants from the Canadian Institutes of Health Research and the Kidney Foundation of Canada (K.A.W. and G.R.). J.C.C. was supported by a traineeship from the Dalhousie Cancer Research Program.
ASJC Scopus Subject Areas
- Immunology and Allergy
- Immunology
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't