Prolonged costimulation is required for naive T cell activation

Robert S. Liwski, Jennifer C. Chase, William H. Baldridge, Irene Sadek, Geoffrey Rowden, Kenneth A. West

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)

Resumen

Costimulation by members of the B7 family of molecules is critical for the activation of naive CD4+ T cells. While prolonged TCR signaling is necessary for T cell activation, the duration of costimulatory signals required has not been established. In this study, murine bone marrow-derived dendritic cells (DC) and naïve CD4+ T cells were used to determine the temporal costimulatory requirements for naive T cell activation. By blocking CD80/CD86 costimulation at various time points during DC-T cell interaction and using the CFSE technique to assess the dynamics of T cell proliferation, we found that prolonged costimulation was required for naive T cells to enter and progress through the cell cycle over a wide range of peptide concentrations. Prolonged costimulation was also important for IL-2 production and CD25/CD69 expression by naive T cells. Video microscopy demonstrated that DC and naive T cells formed stable conjugates that persisted for more than 6 h. Thus, persistent CD80/CD86 signaling during prolonged interactions with DC allows naive T cells to enter the cell cycle and programs the daughter cells to undergo subsequent divisions.

Idioma originalEnglish
Páginas (desde-hasta)135-143
Número de páginas9
PublicaciónImmunology Letters
Volumen106
N.º2
DOI
EstadoPublished - ago. 15 2006

Nota bibliográfica

Funding Information:
Supported by grants from the Canadian Institutes of Health Research and the Kidney Foundation of Canada (K.A.W. and G.R.). J.C.C. was supported by a traineeship from the Dalhousie Cancer Research Program.

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Immunology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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