Resumen
Phagocytosis is a receptor-mediated process whereby professional phagocytes internalize invading pathogens and apoptotic bodies into an intracellular vacuole or phagosome, leading to their degradation. During the formation and maturation of the phagosome, several lipids undergo changes and effector proteins are recruited on the nascent phagosome in a concerted manner. These highly localized, dynamic, and transient processes can only be studied by methods capable of high spatial and temporal resolution. The use of genetically encoded chimeric constructs coupled with fluorescence confocal microscopy enables the continuous, noninvasive analysis of the distribution and metabolism of lipids and effector proteins during phagocytosis. Here, we describe a method where the mouse macrophage cell line, RAW 264.7, and primary macrophages are transiently transfected with fluorescent chimeric probes to analyze and quantify phagocytosis of immunoglobulin-opsonized particles, using confocal microscopy.
Idioma original | English |
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Título de la publicación alojada | Methods in Molecular Biology |
Editorial | Humana Press Inc. |
Páginas | 79-91 |
Número de páginas | 13 |
DOI | |
Estado | Published - 2017 |
Publicado de forma externa | Sí |
Serie de la publicación
Nombre | Methods in Molecular Biology |
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Volumen | 1519 |
ISSN (versión impresa) | 1064-3745 |
Nota bibliográfica
Publisher Copyright:© Springer Science+Business Media New York 2017.
ASJC Scopus Subject Areas
- Molecular Biology
- Genetics
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't