Quantitative live-cell fluorescence microscopy during phagocytosis

Stella M. Lu, Sergio Grinstein, Gregory D. Fairn

Producción científica: Capítulo en Libro/Reporte/Acta de conferenciaCapítulo

11 Citas (Scopus)

Resumen

Phagocytosis is a receptor-mediated process whereby professional phagocytes internalize invading pathogens and apoptotic bodies into an intracellular vacuole or phagosome, leading to their degradation. During the formation and maturation of the phagosome, several lipids undergo changes and effector proteins are recruited on the nascent phagosome in a concerted manner. These highly localized, dynamic, and transient processes can only be studied by methods capable of high spatial and temporal resolution. The use of genetically encoded chimeric constructs coupled with fluorescence confocal microscopy enables the continuous, noninvasive analysis of the distribution and metabolism of lipids and effector proteins during phagocytosis. Here, we describe a method where the mouse macrophage cell line, RAW 264.7, and primary macrophages are transiently transfected with fluorescent chimeric probes to analyze and quantify phagocytosis of immunoglobulin-opsonized particles, using confocal microscopy.

Idioma originalEnglish
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press Inc.
Páginas79-91
Número de páginas13
DOI
EstadoPublished - 2017
Publicado de forma externa

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1519
ISSN (versión impresa)1064-3745

Nota bibliográfica

Publisher Copyright:
© Springer Science+Business Media New York 2017.

ASJC Scopus Subject Areas

  • Molecular Biology
  • Genetics

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

Huella

Profundice en los temas de investigación de 'Quantitative live-cell fluorescence microscopy during phagocytosis'. En conjunto forman una huella única.

Citar esto