TY - JOUR
T1 - Response of mouse proximal straight tubule and medullary thick ascending limb to β-agonist
AU - Morgunov, Nikolas S.
AU - You, Yong Dong
AU - Hirsch, David J.
PY - 1993/11
Y1 - 1993/11
N2 - Scatchard analysis of [3H]CGP-12177 and [125I]cyanopindolol (CYP) radioligand binding data revealed the presence of specific β-adrenergic receptor-binding sites on microdissected mouse proximal straight and medullary thick ascending limb (mTAL) tubules. β-Receptor (10-6 M isoproterenol) stimulation of isolated perfused mTAL tubules produced a consistent hyperpolarization of the transepithelial potential that was blocked by propranolol (10-6 M), a β-receptor antagonist, and furosemide (10-4 M), an inhibitor of the Na+/K+/2 Cl- triporter. In contrast, there was no electrogenic response to isoproterenol stimulation in isolated perfused proximal straight tubules. In summary, radioligand binding data show that both proximal straight and mTAL tubules possess β-receptor-binding sites. Electrogenic transport in the mTAL can be modulated by β-agonists, but there was no detectable electrogenic response to β-receptor stimulation in proximal straight tubules.
AB - Scatchard analysis of [3H]CGP-12177 and [125I]cyanopindolol (CYP) radioligand binding data revealed the presence of specific β-adrenergic receptor-binding sites on microdissected mouse proximal straight and medullary thick ascending limb (mTAL) tubules. β-Receptor (10-6 M isoproterenol) stimulation of isolated perfused mTAL tubules produced a consistent hyperpolarization of the transepithelial potential that was blocked by propranolol (10-6 M), a β-receptor antagonist, and furosemide (10-4 M), an inhibitor of the Na+/K+/2 Cl- triporter. In contrast, there was no electrogenic response to isoproterenol stimulation in isolated perfused proximal straight tubules. In summary, radioligand binding data show that both proximal straight and mTAL tubules possess β-receptor-binding sites. Electrogenic transport in the mTAL can be modulated by β-agonists, but there was no detectable electrogenic response to β-receptor stimulation in proximal straight tubules.
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M3 - Article
C2 - 7905754
AN - SCOPUS:0027688207
SN - 1046-6673
VL - 4
SP - 1151
EP - 1158
JO - Journal of the American Society of Nephrology : JASN
JF - Journal of the American Society of Nephrology : JASN
IS - 5
ER -