Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors

Susan L. Stramer; Cynthia Collins; Thomas Nugent; Xue Wang; Meghan Fuschino; John W. Heitman; Jacqueline Law; David E. Krysztof; Nancy Kiely; Deborah Todd; Nicolaas M.J. Vermeulen; Karen Harrington; Hany Kamel; David J. Kelvin; Michael P. Busch; Kirsten St. George; Indira K. Hewlett; Jeffrey M. Linnen; Philip J. Norris

doi:10.1093/infdis/jir863

Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors

Susan L. Stramer, Cynthia Collins, Thomas Nugent, Xue Wang, Meghan Fuschino, John W. Heitman, Jacqueline Law, David E. Krysztof, Nancy Kiely, Deborah Todd, Nicolaas M.J. Vermeulen, Karen Harrington, Hany Kamel, David J. Kelvin, Michael P. Busch, Kirsten St. George, Indira K. Hewlett, Jeffrey M. Linnen, Philip J. Norris

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26 Citas (Scopus)

Resumen

Background. There have been anecdotal reports of influenza viremia since the 1960s. We present an assessment of the prevalence of seasonal and 2009 H1N1 influenza viremia (via RNA testing) in blood donor populations using multiple sensitive detection assays. Methods. Several influenza RNA amplification assays, including transcription-mediated amplification (TMA) and 2 reverse- transcription polymerase chain reaction (RT-PCR) assays, were evaluated and used to test donor samples. Retrospective samples from 478 subjects drawn at sites with high influenza activity were tested. Prospective samples were collected from 1004 blood donors who called their donation center within 3 days of donation complaining of influenza-like illness (ILI). The plasma collected on the day of donation for these subjects was tested. Results. Of the repository samples, 2 of 478 plasma samples were initially reactive but not repeat reactive by influenza TMA. Of blood donors reporting ILI symptoms postdonation, 1 of 1004 samples was TMA initially reactive but not repeat reactive; all samples were nonreactive by RT-PCR testing. Conclusions. Targeting blood donor populations most likely to have influenza infection, we failed to detect influenza RNA in 1482 donor samples, with most tested by 3 different RNA assays. Seasonal influenza does not appear to pose a significant contamination threat to the blood supply.

Idioma original	English
Páginas (desde-hasta)	886-894
Número de páginas	9
Publicación	Journal of Infectious Diseases
Volumen	205
N.º	6
DOI	https://doi.org/10.1093/infdis/jir863
Estado	Published - mar. 15 2012
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
Acknowledgments. We would like to thank Michael Popowich and Daryl Lamson for excellent technical assistance. And ARC staff, Colleen Winton, Pam Sledge; Ann Kaldun and Beth Hewitt who coordinated and executed obtaining donor samples for testing. The Retrovirus Epidemiology Donor Study–II (REDS-II Study Group) was the responsibility of the following: Blood Centers American Red Cross Blood Services, New England Region R. Cable, J. Rios, and R. Benjamin American Red Cross Blood Services, Southern Region/Department of Pathology and Laboratory Medicine, Emory University School of Medicine J. D. Roback Hoxworth Blood Center, University of Cincinnati Academic Health Center R. A. Sacher, S. L. Wilkinson, and P. M. Carey Blood Centers of the Pacific, University of California San Francisco, Blood Systems Research Institute E. L. Murphy, B. Custer, and N. Hirschler The Institute for Transfusion Medicine D. Triulzi, R. Kakaiya, and J. Kiss Blood Center of Wisconsin J. Gottschall and A. Mast Coordinating Center: Westat, Inc J. Schulman and M. King National Heart, Lung, and Blood Institute, National Institutes of Health G. Nemo Central Laboratory: Blood Systems Research Institute M. P. Busch and P. Norris. Financial support. This work was supported by NHLBI contract N01-HB-57181. Potential conflicts of interest. The following authors are employed at companies: C. C., T. M., K. H., J. M. L. (Gen-Probe), N. M. J. V. (Epoch Biosciences). M. P. B. serves on the scientific advisory board of Gen-Probe. No other authors reported potential conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

ASJC Scopus Subject Areas

Immunology and Allergy
Infectious Diseases

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural

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Stramer, S. L., Collins, C., Nugent, T., Wang, X., Fuschino, M., Heitman, J. W., Law, J., Krysztof, D. E., Kiely, N., Todd, D., Vermeulen, N. M. J., Harrington, K., Kamel, H., Kelvin, D. J., Busch, M. P., St. George, K., Hewlett, I. K., Linnen, J. M., & Norris, P. J. (2012). Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors. Journal of Infectious Diseases, 205(6), 886-894. https://doi.org/10.1093/infdis/jir863

Stramer, SL, Collins, C, Nugent, T, Wang, X, Fuschino, M, Heitman, JW, Law, J, Krysztof, DE, Kiely, N, Todd, D, Vermeulen, NMJ, Harrington, K, Kamel, H, Kelvin, DJ, Busch, MP, St. George, K, Hewlett, IK, Linnen, JM & Norris, PJ 2012, 'Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors', Journal of Infectious Diseases, vol. 205, n.º 6, pp. 886-894. https://doi.org/10.1093/infdis/jir863

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title = "Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors",

abstract = "Background. There have been anecdotal reports of influenza viremia since the 1960s. We present an assessment of the prevalence of seasonal and 2009 H1N1 influenza viremia (via RNA testing) in blood donor populations using multiple sensitive detection assays. Methods. Several influenza RNA amplification assays, including transcription-mediated amplification (TMA) and 2 reverse- transcription polymerase chain reaction (RT-PCR) assays, were evaluated and used to test donor samples. Retrospective samples from 478 subjects drawn at sites with high influenza activity were tested. Prospective samples were collected from 1004 blood donors who called their donation center within 3 days of donation complaining of influenza-like illness (ILI). The plasma collected on the day of donation for these subjects was tested. Results. Of the repository samples, 2 of 478 plasma samples were initially reactive but not repeat reactive by influenza TMA. Of blood donors reporting ILI symptoms postdonation, 1 of 1004 samples was TMA initially reactive but not repeat reactive; all samples were nonreactive by RT-PCR testing. Conclusions. Targeting blood donor populations most likely to have influenza infection, we failed to detect influenza RNA in 1482 donor samples, with most tested by 3 different RNA assays. Seasonal influenza does not appear to pose a significant contamination threat to the blood supply.",

author = "Stramer, {Susan L.} and Cynthia Collins and Thomas Nugent and Xue Wang and Meghan Fuschino and Heitman, {John W.} and Jacqueline Law and Krysztof, {David E.} and Nancy Kiely and Deborah Todd and Vermeulen, {Nicolaas M.J.} and Karen Harrington and Hany Kamel and Kelvin, {David J.} and Busch, {Michael P.} and {St. George}, Kirsten and Hewlett, {Indira K.} and Linnen, {Jeffrey M.} and Norris, {Philip J.}",

note = "Funding Information: Acknowledgments. We would like to thank Michael Popowich and Daryl Lamson for excellent technical assistance. And ARC staff, Colleen Winton, Pam Sledge; Ann Kaldun and Beth Hewitt who coordinated and executed obtaining donor samples for testing. The Retrovirus Epidemiology Donor Study–II (REDS-II Study Group) was the responsibility of the following: Blood Centers American Red Cross Blood Services, New England Region R. Cable, J. Rios, and R. Benjamin American Red Cross Blood Services, Southern Region/Department of Pathology and Laboratory Medicine, Emory University School of Medicine J. D. Roback Hoxworth Blood Center, University of Cincinnati Academic Health Center R. A. Sacher, S. L. Wilkinson, and P. M. Carey Blood Centers of the Pacific, University of California San Francisco, Blood Systems Research Institute E. L. Murphy, B. Custer, and N. Hirschler The Institute for Transfusion Medicine D. Triulzi, R. Kakaiya, and J. Kiss Blood Center of Wisconsin J. Gottschall and A. Mast Coordinating Center: Westat, Inc J. Schulman and M. King National Heart, Lung, and Blood Institute, National Institutes of Health G. Nemo Central Laboratory: Blood Systems Research Institute M. P. Busch and P. Norris. Financial support. This work was supported by NHLBI contract N01-HB-57181. Potential conflicts of interest. The following authors are employed at companies: C. C., T. M., K. H., J. M. L. (Gen-Probe), N. M. J. V. (Epoch Biosciences). M. P. B. serves on the scientific advisory board of Gen-Probe. No other authors reported potential conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.",

year = "2012",

month = mar,

day = "15",

doi = "10.1093/infdis/jir863",

language = "English",

volume = "205",

pages = "886--894",

journal = "Journal of Infectious Diseases",

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TY - JOUR

T1 - Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors

AU - Stramer, Susan L.

AU - Collins, Cynthia

AU - Nugent, Thomas

AU - Wang, Xue

AU - Fuschino, Meghan

AU - Heitman, John W.

AU - Law, Jacqueline

AU - Krysztof, David E.

AU - Kiely, Nancy

AU - Todd, Deborah

AU - Vermeulen, Nicolaas M.J.

AU - Harrington, Karen

AU - Kamel, Hany

AU - Kelvin, David J.

AU - Busch, Michael P.

AU - St. George, Kirsten

AU - Hewlett, Indira K.

AU - Linnen, Jeffrey M.

AU - Norris, Philip J.

N1 - Funding Information: Acknowledgments. We would like to thank Michael Popowich and Daryl Lamson for excellent technical assistance. And ARC staff, Colleen Winton, Pam Sledge; Ann Kaldun and Beth Hewitt who coordinated and executed obtaining donor samples for testing. The Retrovirus Epidemiology Donor Study–II (REDS-II Study Group) was the responsibility of the following: Blood Centers American Red Cross Blood Services, New England Region R. Cable, J. Rios, and R. Benjamin American Red Cross Blood Services, Southern Region/Department of Pathology and Laboratory Medicine, Emory University School of Medicine J. D. Roback Hoxworth Blood Center, University of Cincinnati Academic Health Center R. A. Sacher, S. L. Wilkinson, and P. M. Carey Blood Centers of the Pacific, University of California San Francisco, Blood Systems Research Institute E. L. Murphy, B. Custer, and N. Hirschler The Institute for Transfusion Medicine D. Triulzi, R. Kakaiya, and J. Kiss Blood Center of Wisconsin J. Gottschall and A. Mast Coordinating Center: Westat, Inc J. Schulman and M. King National Heart, Lung, and Blood Institute, National Institutes of Health G. Nemo Central Laboratory: Blood Systems Research Institute M. P. Busch and P. Norris. Financial support. This work was supported by NHLBI contract N01-HB-57181. Potential conflicts of interest. The following authors are employed at companies: C. C., T. M., K. H., J. M. L. (Gen-Probe), N. M. J. V. (Epoch Biosciences). M. P. B. serves on the scientific advisory board of Gen-Probe. No other authors reported potential conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

PY - 2012/3/15

Y1 - 2012/3/15

N2 - Background. There have been anecdotal reports of influenza viremia since the 1960s. We present an assessment of the prevalence of seasonal and 2009 H1N1 influenza viremia (via RNA testing) in blood donor populations using multiple sensitive detection assays. Methods. Several influenza RNA amplification assays, including transcription-mediated amplification (TMA) and 2 reverse- transcription polymerase chain reaction (RT-PCR) assays, were evaluated and used to test donor samples. Retrospective samples from 478 subjects drawn at sites with high influenza activity were tested. Prospective samples were collected from 1004 blood donors who called their donation center within 3 days of donation complaining of influenza-like illness (ILI). The plasma collected on the day of donation for these subjects was tested. Results. Of the repository samples, 2 of 478 plasma samples were initially reactive but not repeat reactive by influenza TMA. Of blood donors reporting ILI symptoms postdonation, 1 of 1004 samples was TMA initially reactive but not repeat reactive; all samples were nonreactive by RT-PCR testing. Conclusions. Targeting blood donor populations most likely to have influenza infection, we failed to detect influenza RNA in 1482 donor samples, with most tested by 3 different RNA assays. Seasonal influenza does not appear to pose a significant contamination threat to the blood supply.

AB - Background. There have been anecdotal reports of influenza viremia since the 1960s. We present an assessment of the prevalence of seasonal and 2009 H1N1 influenza viremia (via RNA testing) in blood donor populations using multiple sensitive detection assays. Methods. Several influenza RNA amplification assays, including transcription-mediated amplification (TMA) and 2 reverse- transcription polymerase chain reaction (RT-PCR) assays, were evaluated and used to test donor samples. Retrospective samples from 478 subjects drawn at sites with high influenza activity were tested. Prospective samples were collected from 1004 blood donors who called their donation center within 3 days of donation complaining of influenza-like illness (ILI). The plasma collected on the day of donation for these subjects was tested. Results. Of the repository samples, 2 of 478 plasma samples were initially reactive but not repeat reactive by influenza TMA. Of blood donors reporting ILI symptoms postdonation, 1 of 1004 samples was TMA initially reactive but not repeat reactive; all samples were nonreactive by RT-PCR testing. Conclusions. Targeting blood donor populations most likely to have influenza infection, we failed to detect influenza RNA in 1482 donor samples, with most tested by 3 different RNA assays. Seasonal influenza does not appear to pose a significant contamination threat to the blood supply.

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JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

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ER -

Sensitive detection assays for influenza RNA do not reveal viremia in US blood donors

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PubMed: MeSH publication types

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