Separation of native and denatured DNA on columns of silk fibroin

T. Y. Huh; C. W. Helleiner

doi:10.1016/0003-2697(67)90144-3

Separation of native and denatured DNA on columns of silk fibroin

T. Y. Huh, C. W. Helleiner

Medicine

Medicine

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

Columns of suitably pretreated silk fibroin adsorb denatured DNA from 0.2 M MgCl₂ solution, but allow unretarded passage of native DNA. Denatured DNA can be eluted from such columns with dilute phosphate buffer. These columns can be used to separate native and denatured DNA. The capacity of the columns is about 1 mg of denatured DNA per gram of fibroin.

Idioma original	English
Páginas (desde-hasta)	150-156
Número de páginas	7
Publicación	Analytical Biochemistry
Volumen	19
N.º	1
DOI	https://doi.org/10.1016/0003-2697(67)90144-3
Estado	Published - abr. 1967

Nota bibliográfica

Funding Information:
This work was supported by grants from the Medical Research Council of Canada, The National Cancer Institute of Canada, and the Banting Research Foundation. We are also grateful to Dr. H. Kojima for a sample of silk for the early stages of this work, and to Mr. M. E. Zinck for the preparation of deoxyadenylate-deoxythymidylate copolymer.

ASJC Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

PubMed: MeSH publication types

Journal Article

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10.1016/0003-2697(67)90144-3

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title = "Separation of native and denatured DNA on columns of silk fibroin",

abstract = "Columns of suitably pretreated silk fibroin adsorb denatured DNA from 0.2 M MgCl2 solution, but allow unretarded passage of native DNA. Denatured DNA can be eluted from such columns with dilute phosphate buffer. These columns can be used to separate native and denatured DNA. The capacity of the columns is about 1 mg of denatured DNA per gram of fibroin.",

author = "Huh, {T. Y.} and Helleiner, {C. W.}",

note = "Funding Information: This work was supported by grants from the Medical Research Council of Canada, The National Cancer Institute of Canada, and the Banting Research Foundation. We are also grateful to Dr. H. Kojima for a sample of silk for the early stages of this work, and to Mr. M. E. Zinck for the preparation of deoxyadenylate-deoxythymidylate copolymer.",

year = "1967",

month = apr,

doi = "10.1016/0003-2697(67)90144-3",

language = "English",

volume = "19",

pages = "150--156",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

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T1 - Separation of native and denatured DNA on columns of silk fibroin

AU - Huh, T. Y.

AU - Helleiner, C. W.

N1 - Funding Information: This work was supported by grants from the Medical Research Council of Canada, The National Cancer Institute of Canada, and the Banting Research Foundation. We are also grateful to Dr. H. Kojima for a sample of silk for the early stages of this work, and to Mr. M. E. Zinck for the preparation of deoxyadenylate-deoxythymidylate copolymer.

PY - 1967/4

Y1 - 1967/4

N2 - Columns of suitably pretreated silk fibroin adsorb denatured DNA from 0.2 M MgCl2 solution, but allow unretarded passage of native DNA. Denatured DNA can be eluted from such columns with dilute phosphate buffer. These columns can be used to separate native and denatured DNA. The capacity of the columns is about 1 mg of denatured DNA per gram of fibroin.

AB - Columns of suitably pretreated silk fibroin adsorb denatured DNA from 0.2 M MgCl2 solution, but allow unretarded passage of native DNA. Denatured DNA can be eluted from such columns with dilute phosphate buffer. These columns can be used to separate native and denatured DNA. The capacity of the columns is about 1 mg of denatured DNA per gram of fibroin.

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U2 - 10.1016/0003-2697(67)90144-3

DO - 10.1016/0003-2697(67)90144-3

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AN - SCOPUS:0014072636

SN - 0003-2697

VL - 19

SP - 150

EP - 156

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

Separation of native and denatured DNA on columns of silk fibroin

Resumen

Nota bibliográfica

ASJC Scopus Subject Areas

PubMed: MeSH publication types

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