Sequence analysis of the cloned streptococcal cell surface antigen I/II

C. Kelly; P. Evans; L. Bergmeier; S. F. Lee; A. Progulske-Fox; A. C. Hams; A. Aitken; A. S. Bleiweis; T. Lehner

doi:10.1016/0014-5793(89)81632-1

Sequence analysis of the cloned streptococcal cell surface antigen I/II

C. Kelly, P. Evans, L. Bergmeier, S. F. Lee, A. Progulske-Fox, A. C. Hams, A. Aitken, A. S. Bleiweis, T. Lehner

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Resumen

The gene spa P (formerly designated as spa P1) encoding the M_r 185,000 surface antigen (I/II) of Streptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequences. The N-terminal region (60-550) has alanine-rich repeats and is predicted to be α-helical. However, the C-ternunal region (800-1540) is proline-rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N-Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N-terminal and the latter the C-terminal portions of the intact antigen.

Idioma original	English
Páginas (desde-hasta)	127-132
Número de páginas	6
Publicación	FEBS Letters
Volumen	258
N.º	1
DOI	https://doi.org/10.1016/0014-5793(89)81632-1
Estado	Published - nov. 20 1989
Publicado de forma externa	Sí

Nota bibliográfica

Funding Information:
AcknowledgementsT:h is work was supportedb y The Dickinson Trust and by US Public Health Service,G rant R37-DE-08007to A.S.B. from the National Institute of Dental Research, NIH, Bethesda,M D, USA.

ASJC Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Sequence analysis of the cloned streptococcal cell surface antigen I/II",

abstract = "The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequences. The N-terminal region (60-550) has alanine-rich repeats and is predicted to be α-helical. However, the C-ternunal region (800-1540) is proline-rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N-Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N-terminal and the latter the C-terminal portions of the intact antigen.",

author = "C. Kelly and P. Evans and L. Bergmeier and Lee, {S. F.} and A. Progulske-Fox and Hams, {A. C.} and A. Aitken and Bleiweis, {A. S.} and T. Lehner",

note = "Funding Information: AcknowledgementsT:h is work was supportedb y The Dickinson Trust and by US Public Health Service,G rant R37-DE-08007to A.S.B. from the National Institute of Dental Research, NIH, Bethesda,M D, USA.",

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T1 - Sequence analysis of the cloned streptococcal cell surface antigen I/II

AU - Kelly, C.

AU - Evans, P.

AU - Bergmeier, L.

AU - Lee, S. F.

AU - Progulske-Fox, A.

AU - Hams, A. C.

AU - Aitken, A.

AU - Bleiweis, A. S.

AU - Lehner, T.

N1 - Funding Information: AcknowledgementsT:h is work was supportedb y The Dickinson Trust and by US Public Health Service,G rant R37-DE-08007to A.S.B. from the National Institute of Dental Research, NIH, Bethesda,M D, USA.

PY - 1989/11/20

Y1 - 1989/11/20

N2 - The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequences. The N-terminal region (60-550) has alanine-rich repeats and is predicted to be α-helical. However, the C-ternunal region (800-1540) is proline-rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N-Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N-terminal and the latter the C-terminal portions of the intact antigen.

AB - The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutons, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequences. The N-terminal region (60-550) has alanine-rich repeats and is predicted to be α-helical. However, the C-ternunal region (800-1540) is proline-rich and favours an extended structure. Except for a short central variable region the sequences appear to be highly conserved for S. mutans serotype c. N-Tenninal sequencing of separated antigen I and antigen II polypeptides suggests that the former represents the N-terminal and the latter the C-terminal portions of the intact antigen.

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Sequence analysis of the cloned streptococcal cell surface antigen I/II

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ASJC Scopus Subject Areas

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