Resumen
The basic fibroblast growth factor (FGF-2) gene is transcribed bidirectionally to yield multiple sense (coding) transcripts and a unique 1.5 kb antisense transcript which may regulate sense RNA stability. The antisense RNA also contains a long open reading frame that predicts a hypothetical protein with homology to the prokaryotic MutT antimutator proteins. However, translation of this protein has not previously been demonstrated. We employed antibodies against the conserved MutT-domain of the deduced human FGF-2 antisense protein (GFG) to demonstrate expression of an immunoreactive 24 kDa protein in liver extracts from Xenopus laevis, and two proteins of 28 and 35 kDa in rat liver. In rats, GFG protein expression detected by western blot was tissue-specific and correlated with the level of FGF-2 antisense mRNA expression. These findings demonstrate that, in addition to its possible RNA regulatory function, the FGF-2 antisense transcript is translated into a conserved MutT-related protein.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 19-23 |
| Número de páginas | 5 |
| Publicación | Biochemical and Biophysical Research Communications |
| Volumen | 223 |
| N.º | 1 |
| DOI | |
| Estado | Published - jun. 5 1996 |
Nota bibliográfica
Funding Information:We are grateful to Dr. David Kimelman (University of Washington) for the XF11 cDNA and to Dr. Richard Wassersug (Dalhousie University) for providing Xenopus laevis tissues for this study. Critical reading of the manuscript by Dr. W. H. Moger is greatly appreciated. This research was supported by grants to P.R.M. from the Medical Research Council of Canada and the Atlee Endowment.
ASJC Scopus Subject Areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't