The role of V3 neurons in speeddependent interlimb coordination during locomotion in mice

Han Zhang, Natalia A. Shevtsova, Dylan Deska-Gauthier, Colin Mackay, Kimberly J. Dougherty, Simon M. Danner, Ying Zhang, Ilya A. Rybak

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

17 Citas (Scopus)

Resumen

Speed-dependent interlimb coordination allows animals to maintain stable locomotion under different circumstances. The V3 neurons are known to be involved in interlimb coordination. We previously modeled the locomotor spinal circuitry controlling interlimb coordination (Danner et al., 2017). This model included the local V3 neurons that mediate mutual excitation between left and right rhythm generators (RGs). Here, our focus was on V3 neurons involved in ascending long propriospinal interactions (aLPNs). Using retrograde tracing, we revealed a subpopulation of lumbar V3 aLPNs with contralateral cervical projections. V3OFF mice, in which all V3 neurons were silenced, had a significantly reduced maximal locomotor speed, were unable to move using stable trot, gallop, or bound, and predominantly used a lateral-sequence walk. To reproduce this data and understand the functional roles of V3 aLPNs, we extended our previous model by incorporating diagonal V3 aLPNs mediating inputs from each lumbar RG to the contralateral cervical RG. The extended model reproduces our experimental results and suggests that locally projecting V3 neurons, mediating left– right interactions within lumbar and cervical cords, promote left–right synchronization necessary for gallop and bound, whereas the V3 aLPNs promote synchronization between diagonal fore and hind RGs necessary for trot. The model proposes the organization of spinal circuits available for future experimental testing.

Idioma originalEnglish
Número de artículoe73424
PublicacióneLife
Volumen11
DOI
EstadoPublished - abr. 2022

Nota bibliográfica

Funding Information:
The authors thank Dr. Ole Kiehn for useful comments and suggestions and Dr. Joanna Borowska-Fielding, Mr. Dallas Bennett, Mr. Ramez Michail, and Mr. Igor Tatarnikov for technical support. This work was supported by the grants of the National Institutes of Health: R01 NS104194 (KJD), R01 NS110550 (IAR), R01 NS112304 (SMD), R01 NS115900 (SMD), and R21 NS118226 (KJD); the National Science Foundation: grant # 2113069 (IAR); the Canadian Institutes of Health Research: MOP110950 (YZ) and PJT-173547 (YZ); and the Natural Sciences and Engineering Research Council of Canada: RGPIN 04880 (YZ).

Funding Information:
The authors thank Dr. Ole Kiehn for useful comments and suggestions and Dr. Joanna BorowskaFielding, Mr. Dallas Bennett, Mr. Ramez Michail, and Mr. Igor Tatarnikov for technical support. This work was supported by the grants of the National Institutes of Health: R01 NS104194 (KJD), R01 NS110550 (IAR), R01 NS112304 (SMD), R01 NS115900 (SMD), and R21 NS118226 (KJD); the National Science Foundation: grant # 2113069 (IAR); the Canadian Institutes of Health Research: MOP110950 (YZ) and PJT-173547 (YZ); and the Natural Sciences and Engineering Research Council of Canada: RGPIN 04880 (YZ). Funding Funder Grant reference number Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health R01NS104194 R21NS118226 R01NS112304 R01NS115900 R01NS110550 Kimberly J Dougherty Kimberly J Dougherty Simon M Danner Simon M Danner Ilya A Rybak National Science Foundation 2113069 Ilya A Rybak Canadian Institutes of Health Research Canadian Institutes of Health Research Natural Sciences and Engineering Research Council of Canada MOP110950 PJT-173547 RGPIN 04880 Ying Zhang Ying Zhang Ying Zhang The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Publisher Copyright:
‍‍© Zhang et al.

ASJC Scopus Subject Areas

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

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