Resumen
Perforation formation in Aponogeton madagascariensis (Mirb.) H.Bruggen (lace plant) is an excellent model for studying developmentally regulated programmed cell death (PCD). In this study, we isolated and identified two lace plant vacuolar processing enzymes (VPEs) and investigated their involvement in PCD and throughout leaf development. Lace plant VPE transcript levels were determined during seven different stages of leaf development. PCD and non-PCD cells from “window” stage leaves (in which perforations are forming) were separated through laser-capture microscopy and their transcript levels were also determined. VPE activity was also studied between the cell types, through a VPE activity-based probe JOPD1. Additionally, VPE transcript levels were studied in plants treated with an ethylene biosynthesis inhibitor, aminoethoxyvinylglycine (AVG). The two isolated VPEs, AmVPE1 and AmVPE2, are vegetative type VPEs. AmVPE1 had higher transcript levels during a pre-perforation developmental stage, immediately prior to visible signs of PCD. AmVPE2 transcript levels were higher later during window and late window stages. Both VPEs had higher transcript and activity levels in PCD compared with the non-PCD cells. AVG treatment inhibited PCD and associated increases in VPE transcript levels. Our results suggested that VPEs are involved in the execution of the ethylene-related PCD in the lace plant.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 235-247 |
| Número de páginas | 13 |
| Publicación | Botany |
| Volumen | 96 |
| N.º | 4 |
| DOI | |
| Estado | Published - 2018 |
Nota bibliográfica
Funding Information:The authors acknowledge the Natural Sciences and Engineering Research Council of Canada (NSERC, grant No. 45162) and the Canada Foundation for Innovation (CFI, grant No. 14831) for providing funding to A.H.L.A.N.G. We also thank the Botswana government and Botswana International University of Science and Technology (BIUST) for providing Graduate scholarship funding to G.R. The authors also thank Dr. Julie Kang (University of Northern Iowa, USA) and Dr. Adrian Dauphinee (Dalhou-sie University) for critically reviewing this manuscript. We thank Dr. Renier van der Hoorn (Department of Plant Sciences, University of Oxford, UK) for providing the VPE activity-based probe. Gaolathe Rantong carried out all of the experiments, wrote the first manuscript and made final manuscript revisions. Arunika Gunawardena conceived the study, participated in its design and coordination, and helped with manuscript revisions as well as supervising all of the experimental work.
Funding Information:
The authors acknowledge the Natural Sciences and Engineering Research Council of Canada (NSERC, grant No. 45162) and the Canada Foundation for Innovation (CFI, grant No. 14831) for providing funding to A.H.L.A.N.G. We also thank the Botswana government and Botswana International University of Science and Technology (BIUST) for providing Graduate scholarship funding to G.R. The authors also thank Dr. Julie Kang (University of Northern Iowa, USA) and Dr. Adrian Dauphinee (Dalhousie University) for critically reviewing this manuscript. We thank Dr. Renier van der Hoorn (Department of Plant Sciences, University of Oxford, UK) for providing the VPE activity-based probe. Gaolathe Rantong carried out all of the experiments, wrote the first manuscript and made final manuscript revisions. Arunika Gunawardena conceived the study, participated in its design and coordination, and helped with manuscript revisions as well as supervising all of the experimental work.
Publisher Copyright:
© 2018, Canadian Science Publishing. All rights reserved.
ASJC Scopus Subject Areas
- Ecology, Evolution, Behavior and Systematics
- Ecology
- Plant Science