Taxonomic study of a Japanese native grape cultivar 'Koshu' through SNPs analysis and partial sequence of chloroplast DNA

Outline of Final Research Achievements

'Koshu' is a Japanese native grape cultivar and had been believed to be an oriental variety of Vitis vinifera; however, there were different opinions. Thus, to clarify the taxonomic position of 'Koshu', we carried out polymorphic DNA analyses. The result of single nucleotide polymorphisms (SNPs) analysis indicated that genomic DNA of 'Koshu' consists of slightly more than 70% of V. vinifera and slightly less than 30% of Asian wild species. In addition, the chloroplast DNA, which is inherited from the maternal ancestor, of 'Koshu' is a wild type and most closely related to that of a Chinese wild speciesV. davidii. From these results, it was revealed that 'Koshu' is a hybrid cultivar with V. davidii or closely related species as the maternal ancestor, even though the percentage of V. vinifera is higher than that of wild species.