Analysis of silver-stained proteins in interphase condensed chromatin in root-tip meristematic cells of common wheat

By using an "en bloc" silver-staining method, it was observed under the transmission electron microscope that there were various amounts of silver-stained granules of different sizes distributed in the interphase condensed chromatin. The results analyzed by image analysis instrument indicated that the shape of the larger granules and some of the smaller granules looked like dots but the others were short fibers. From the results, it could be concluded that nonhistone protein scaffold existed in the condensed chromatin of root meristematic cell in common wheat, the scaffold proteins were distributed over the whole condensed chromatin in the form of granules and fibers. The relationship between chromatin scaffold, chromosome scaffold and nuclear matrix was discussed.