Assessment of Hypoxia and TNF-α response by a vector with HRE and NF-kappaB response elements

Zhilin Chen, Ashley L. Eadie, Sean R. Hall, Laurel Ballantyne, David Ademidun, M. Yat Tse, Stephen C. Pang, Luis G. Melo, Christopher A. Ward, Keith R. Brunt

Résultat de recherche: Articleexamen par les pairs

3 Citations (Scopus)

Résumé

Hypoxia and inflammatory cytokine activation (H&I) are common processes in many acute and chronic diseases. Thus, a single vector that responds to both hypoxia and inflammatory cytokines, such as TNF-α, is useful for assesing the severity of such diseases. Adaptation to hypoxia is regulated primarily by hypoxia inducible transcription factor (HIFα) nuclear proteins that engage genes containing a hypoxia response element (HRE). Inflammation activates a multitude of cytokines, including TNF-α, that invariably modulate activation of the nuclear factor kappa B (NF-kB) transcription factor. We constructed a vector that encompassed both a hypoxia response element (HRE), and a NF-kappaB responsive element. We show that this vector was functionally responsive to both hypoxia and TNF-α, in vitro and in vivo. Thus, this vector might be suitable for the detection and assessment of hypoxia or TNF-α.

Langue d'origineEnglish
Pages (de-à)46-54
Nombre de pages9
JournalFrontiers in Bioscience - Scholar
Volume9
Numéro de publication1
DOI
Statut de publicationPublished - janv. 1 2017

ASJC Scopus Subject Areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Immunology and Microbiology

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Citer

Chen, Z., Eadie, A. L., Hall, S. R., Ballantyne, L., Ademidun, D., Tse, M. Y., Pang, S. C., Melo, L. G., Ward, C. A., & Brunt, K. R. (2017). Assessment of Hypoxia and TNF-α response by a vector with HRE and NF-kappaB response elements. Frontiers in Bioscience - Scholar, 9(1), 46-54. https://doi.org/10.2741/S471