Biophysical characterization of G-protein coupled receptor-peptide ligand binding

David N. Langelaan, Pascaline Ngweniform, Jan K. Rainey

Résultat de recherche: Review articleexamen par les pairs

12 Citations (Scopus)

Résumé

G-protein coupled receptors (GPCRs) are ubiquitous membrane proteins allowing intracellular responses to extracellular factors that range from photons of light to small molecules to proteins. Despite extensive exploitation of GPCRs as therapeutic targets, biophysical characterization of GPCR-ligand interactions remains challenging. In this minireview, we focus on techniques that have been successfully used for structural and biophysical characterization of peptide ligands binding to their cognate GPCRs. The techniques reviewed include solution-state nuclear magnetic resonance (NMR) spectroscopy, solid-state NMR, X-ray diffraction, fluorescence spectroscopy and single-molecule fluorescence methods, flow cytometry, surface plasmon resonance, isothermal titration calorimetry, and atomic force microscopy. The goal herein is to provide a cohesive starting point to allow selection of techniques appropriate to the elucidation of a given GPCR-peptide interaction.

Langue d'origineEnglish
Pages (de-à)98-105
Nombre de pages8
JournalBiochemistry and Cell Biology
Volume89
Numéro de publication2
DOI
Statut de publicationPublished - avr. 2011

ASJC Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Review

Empreinte numérique

Plonger dans les sujets de recherche 'Biophysical characterization of G-protein coupled receptor-peptide ligand binding'. Ensemble, ils forment une empreinte numérique unique.

Citer