Ca2+ and the sarcoplasmic reticulum

Winifred G. Nayler; P. E. Dresel

doi:10.1016/S0022-2828(84)80705-1

Ca²⁺ and the sarcoplasmic reticulum

Winifred G. Nayler, P. E. Dresel

Résultat de recherche: Article › examen par les pairs

4 Citations (Scopus)

Résumé

The role of the sarcoplasmic reticulum in the regulation of cytosolic calcium is briefly reviewed. A method is described for monitoring calcium efflux from relatively large amounts of SR in a newly designed filtration apparatus using membrane filtration. Two phases of Ca²⁺ efflux from SR preloaded in the presence of oxalate were observed: an early exponential phase, followed by a phase of constant efflux. Changes in pH from 6.8 to 7.4 resulted in increased efflux. A change in the opposite direction resulted in an initial rapid increase in efflux before the expected decrease occurred. Caffeine (10 and 20 mm) caused increased efflux. Addition of 100 μm EGTA to the buffer resulted in major changes in the exponential phase but not in the phase of constant release, and potentiated the effect of caffeine. The method appears suitable for a variety of pharmacological studies in SR as well as other tissue fractions.

Langue d'origine	English
Pages (de-à)	165-174
Nombre de pages	10
Journal	Journal of Molecular and Cellular Cardiology
Volume	16
Numéro de publication	2
DOI	https://doi.org/10.1016/S0022-2828(84)80705-1
Statut de publication	Published - févr. 1984
Publié à l'externe	Oui

Note bibliographique

Funding Information:
* Supported by grants from the National Health and Medical Research Council of Australia and the Medical Research Council of Canada. ** To whom requests for offprints should be addressed. t Visiting Research Associate, Presenta ddress: Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, N.S., Canada.

ASJC Scopus Subject Areas

Molecular Biology
Cardiology and Cardiovascular Medicine

PubMed: MeSH publication types

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Review

Accès au document

10.1016/S0022-2828(84)80705-1

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title = "Ca2+ and the sarcoplasmic reticulum",

abstract = "The role of the sarcoplasmic reticulum in the regulation of cytosolic calcium is briefly reviewed. A method is described for monitoring calcium efflux from relatively large amounts of SR in a newly designed filtration apparatus using membrane filtration. Two phases of Ca2+ efflux from SR preloaded in the presence of oxalate were observed: an early exponential phase, followed by a phase of constant efflux. Changes in pH from 6.8 to 7.4 resulted in increased efflux. A change in the opposite direction resulted in an initial rapid increase in efflux before the expected decrease occurred. Caffeine (10 and 20 mm) caused increased efflux. Addition of 100 μm EGTA to the buffer resulted in major changes in the exponential phase but not in the phase of constant release, and potentiated the effect of caffeine. The method appears suitable for a variety of pharmacological studies in SR as well as other tissue fractions.",

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note = "Funding Information: * Supported by grants from the National Health and Medical Research Council of Australia and the Medical Research Council of Canada. ** To whom requests for offprints should be addressed. t Visiting Research Associate, Presenta ddress: Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, N.S., Canada.",

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N2 - The role of the sarcoplasmic reticulum in the regulation of cytosolic calcium is briefly reviewed. A method is described for monitoring calcium efflux from relatively large amounts of SR in a newly designed filtration apparatus using membrane filtration. Two phases of Ca2+ efflux from SR preloaded in the presence of oxalate were observed: an early exponential phase, followed by a phase of constant efflux. Changes in pH from 6.8 to 7.4 resulted in increased efflux. A change in the opposite direction resulted in an initial rapid increase in efflux before the expected decrease occurred. Caffeine (10 and 20 mm) caused increased efflux. Addition of 100 μm EGTA to the buffer resulted in major changes in the exponential phase but not in the phase of constant release, and potentiated the effect of caffeine. The method appears suitable for a variety of pharmacological studies in SR as well as other tissue fractions.

AB - The role of the sarcoplasmic reticulum in the regulation of cytosolic calcium is briefly reviewed. A method is described for monitoring calcium efflux from relatively large amounts of SR in a newly designed filtration apparatus using membrane filtration. Two phases of Ca2+ efflux from SR preloaded in the presence of oxalate were observed: an early exponential phase, followed by a phase of constant efflux. Changes in pH from 6.8 to 7.4 resulted in increased efflux. A change in the opposite direction resulted in an initial rapid increase in efflux before the expected decrease occurred. Caffeine (10 and 20 mm) caused increased efflux. Addition of 100 μm EGTA to the buffer resulted in major changes in the exponential phase but not in the phase of constant release, and potentiated the effect of caffeine. The method appears suitable for a variety of pharmacological studies in SR as well as other tissue fractions.

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