Résumé
High levels of expression of the glucose transporter (GLUT) isoforms 1 and 3 have been demonstrated in the uman placenta by Northern blotting. However, the cellular localization of placental GLUT mRNA has not been described. Furthermore, recent preliminary kinetic data indicate that GLUT 2 might be present in syncytiotrophoblast. Human placental tissue from preterm (16-22 weeks) and term pregnancies was collected for identification and localization of glucose transporter mRNA. Following paraffin embedding, sections were cut and in situ hybridization was performed with fluorescein-labelled cRNA. In addition, immunoblotting and immunocytochemistry were carried out using an anti-GLUT 2 antibody. GLUT 1 mRNA was highly expressed in syncytiotrophoblast cells at term. GLUT 1 expression was much less abundant in non-syncytial cells. In contrast, GLUT 3 mRNA was present in lower amounts and more evenly distributed between syncytial and other placental cells. GLUT 1 mRNA was also highly abundant in preterm syncytiotrophoblast. The cellular distributions of GLUT 1 and GLUT 3 mRNA in the preterm placentas were similar to those in term tissue. With regard to GLUT 1, these findings correlate well with cellular localization and gestational development of GLUT 1 protein. No GLUT 2 protein was detected. It is concluded that GLUT 1 is the main isoform involved in transplacental glucose transport in the human.
Langue d'origine | English |
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Pages (de-à) | 1425-1430 |
Nombre de pages | 6 |
Journal | Reproduction, Fertility and Development |
Volume | 7 |
Numéro de publication | 6 |
DOI | |
Statut de publication | Published - 1995 |
Publié à l'externe | Oui |
Note bibliographique
Funding Information:We thank the staff of the labour and delivery wards at University of California Medical Center, San Francisco, for help in obtaining placental tissue. The skilled technical assistance of Cheryl Malcamp is gratefully acknowledged. This work was supported by a March of Dimes Basic Research Grant to N.P.I. T.J. was supported in part by grants from the Henning and Johan Throne-Holsts Foundation, Swedish Medical Society and Swedish Medical Research Council (Project no B94-04X-10838-01A).
ASJC Scopus Subject Areas
- Biotechnology
- Reproductive Medicine
- Animal Science and Zoology
- Molecular Biology
- Genetics
- Endocrinology
- Developmental Biology