Cellular retinol-binding protein type II (CRBPII) in adult zebrafish (Danio rerio): cDNA sequence, tissue-specific expression and gene linkage analysis

Marianne C. Cameron, Eileen M. Denovan-Wright, Mukesh K. Sharma, Jonathan M. Wright

Résultat de recherche: Articleexamen par les pairs

12 Citations (Scopus)

Résumé

We have determined the nucleotide sequence of a zebrafish cDNA clone that codes for a cellular retinol-binding protein type II (CRBPII). Radiation hybrid mapping revealed that the zebrafish and human CRBPII genes are located in syntenic groups. In situ hybridization and emulsion autoradiography localized the CRBPII mRNA to the intestine and the liver of adult zebrafish. CRBPII and intestinal fatty acid binding protein (I-FABP) mRNA was colocalized to the same regions along the anterior-posterior gradient of the zebrafish intestine. Similarly, CRBPII and I-FABP mRNA are colocalized in mammalian and chicken intestine. CRBPII mRNA, but not I-FABP mRNA, was detected in adult zebrafish liver which is in contrast to mammals where liver CRBPII mRNA levels are high during development but rapidly decrease to very low or undetectable levels following birth. CRBPII and I-FABP gene expression appears therefore to be co-ordinately regulated in the zebrafish intestine as has been suggested for mammals and chicken, but CRBPII gene expression is markedly different in the liver of adult zebrafish compared to the livers of mammals. As such, retinol metabolism in zebrafish may differ from that of mammals and require continued production of CRBPII in adult liver. The primary sequence of the coding regions of fish and mammalian CRBPII genes, their relative chromosomal location in syntenic groups and possibly portions of the control regions involved in regulation of CRBPII gene expression in the intestine appear therefore to have been conserved for more than 400 million years.

Langue d'origineEnglish
Pages (de-à)4685-4692
Nombre de pages8
JournalEuropean Journal of Biochemistry
Volume269
Numéro de publication18
DOI
Statut de publicationPublished - 2002

ASJC Scopus Subject Areas

  • Biochemistry

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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