Characterization and tissue-specific expression of the rat basic fibroblast growth factor antisense mRNA and protein

Rai Knee, Audrey Li, Paul R. Murphy

Résultat de recherche: Articleexamen par les pairs

49 Citations (Scopus)

Résumé

An RNA transcribed from the antisense strand of the FGF-2 gene has been implicated in the regulation of FGF-2 mRNA stability in amphibian oocytes. We have now cloned and characterized a novel 1.1-kb mRNA (fgf-as) from neonatal rat liver. In non-central nervous system (CNS) tissues the fgf-as RNA is abundantly expressed in a developmentally regulated manner. The FGF-AS cDNA contains a consensus polyadenylylation signal and a long open reading frame (ORF) whose deduced amino acid sequence predicts a 35-kDa protein with homology to the Mutt family of nucleotide hydrolases. Western blot analysis with antibodies against the deduced peptide sequence demonstrates that the FGF-AS protein is expressed in a broad range of non-CNS tissue in the postnatal period. In the developing brain, the abundance of sense and antisense transcripts are inversely related, suggesting a role for the antisense RNA in posttranscriptional regulation of FGF-2 expression in this tissue. The FGF-AS is complementary to two widely separated regions in the long 3' untranslated region of the FGF-2 mRNA, in the vicinity of the proximal and distal polyadenylylation sites. These findings demonstrate that the FGF-2 and fgf-as RNAs are coordinately transcribed on a tissue-specific and developmentally regulated basis and suggest that interaction of the sense and antisense RNAs may result in posttranscriptional regulation of FGF-2 in some tissues.

Langue d'origineEnglish
Pages (de-à)4943-4947
Nombre de pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Numéro de publication10
DOI
Statut de publicationPublished - mai 13 1997

ASJC Scopus Subject Areas

  • General

PubMed: MeSH publication types

  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

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Citer

Knee, R., Li, A., & Murphy, P. R. (1997). Characterization and tissue-specific expression of the rat basic fibroblast growth factor antisense mRNA and protein. Proceedings of the National Academy of Sciences of the United States of America, 94(10), 4943-4947. https://doi.org/10.1073/pnas.94.10.4943