Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner

Katsuya Tsuchihara, Valentina Lapin, Christopher Bakal, Hitoshi Okada, Lauren Brown, Masami Hirota-Tsuchihara, Kathrin Zaugg, Alexandra Ho, Annick Itie-YouTen, Marees Harris-Brandts, Robert Rottapel, Christopher D. Richardson, Samuel Benchimol, Tak Wah Mak

Résultat de recherche: Articleexamen par les pairs

42 Citations (Scopus)

Résumé

We used DNA microarray screening to identify Ckap2 (cytoskeleton associated protein 2) as a novel p53 target gene in a mouse erythroleukemia cell line. DNA damage induces human and mouse CKAP2 expression in a p53-dependent manner and p53 activates the Ckap2 promoter. Overexpressed Ckap2 colocalizes with and stabilizes microtubules. In p53-null cells, overexpression of Ckap2 induces tetraploidy with aberrant centrosome numbers, suggesting disturbed mitosis and cytokinesis. In p53-competent cells, Ckap2 does not induce tetraploidy but activates p53-mediated cell cycle arrest and apoptosis. Our data suggest the existence of a functional positive feedback loop in which Ckap2 activates the G1 tetraploidy checkpoint and prevents aneuploidy.

Langue d'origineEnglish
Pages (de-à)6685-6691
Nombre de pages7
JournalCancer Research
Volume65
Numéro de publication15
DOI
Statut de publicationPublished - août 1 2005
Publié à l'externeOui

ASJC Scopus Subject Areas

  • Oncology
  • Cancer Research

Empreinte numérique

Plonger dans les sujets de recherche 'Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner'. Ensemble, ils forment une empreinte numérique unique.

Citer