Résumé
The mucus layer on the surface of fish consists of several antimicrobial agents that provide a first line of defense against invading pathogens. To date, little is known about the antimicrobial properties of the mucus of Arctic char (Salvelinus alpinus), brook trout (S. fontinalis), koi carp (Cyprinus carpio sub sp. koi), striped bass (Morone saxatilis), haddock (Melanogrammus aeglefinus) and hagfish (Myxine glutinosa). The epidermal mucus samples from these fish were extracted with acidic, organic and aqueous solvents to identify potential antimicrobial agents including basic peptides, secondary metabolites, aqueous and acid soluble compounds. Initial screening of the mucus extracts against a susceptible strain of Salmonella enterica C610, showed a significant variation in antimicrobial activity among the fish species examined. The acidic mucus extracts of brook trout, haddock and hagfish exhibited bactericidal activity. The organic mucus extracts of brook trout, striped bass and koi carp showed bacteriostatic activity. There was no detectable activity in the aqueous mucus extracts. Further investigations of the activity of the acidic mucus extracts of brook trout, haddock and hagfish showed that these fish species had specific activity for fish and human pathogens, demonstrating the role of fish mucus in antimicrobial protection. In comparison to brook trout and haddock, the minimum bactericidal concentrations of hagfish acidic mucus extracts were found to be ∼ 1.5 to 3.0 times lower against fish pathogens and ∼ 1.6 to 6.6 folds lower for human pathogens. This preliminary information suggests that the mucus from these fish species may be a source of novel antimicrobial agents for fish and human health related applications. Crown
| Langue d'origine | English |
|---|---|
| Pages (de-à) | 85-92 |
| Nombre de pages | 8 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 150 |
| Numéro de publication | 1 |
| DOI | |
| Statut de publication | Published - mai 2008 |
| Publié à l'externe | Oui |
Note bibliographique
Funding Information:The authors would like to thank Dr. Jim Duston (Nova Scotia Agricultural College, Truro, NS) and Mr. Eric MacKinnon (Cape Sable Island, NS) for providing the fish species; Ron Melanson, Aaron Dumas, Kirty Solanky and Alaina Boyd for their help with sampling; Cheryl Craft and Dr. Roger Ebanks for their assistance in mucus extraction and antimicrobial assays used in this study. Dr. Vanya K. Ewart, Dr. Stewart Johnson and Dr. Susan E. Douglas for providing the bacterial cultures. This research was funded by the National Research Council Canada and the Ford Foundation International Fellowship Program.
ASJC Scopus Subject Areas
- Biochemistry
- Physiology
- Molecular Biology
ODD de l’ONU
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