Cutting Edge: Dendritic Cell Actin Cytoskeletal Polarization during Immunological Synapse Formation Is Highly Antigen-Dependent

Monther M. Al-Alwan, Robert S. Liwski, S. M.Mansour Haeryfar, William H. Baldridge, David W. Hoskin, Geoffrey Rowden, Kenneth A. West

Résultat de recherche: Articleexamen par les pairs

92 Citations (Scopus)

Résumé

Dendritic cells (DC) actively rearrange their actin cytoskeleton to participate information of the immunological synapse (IS). In this study, we evaluated the requirements for DC participation in the IS. DC rearrange their actin cytoskeleton toward naive CD4+ T cells only in the presence of specific MHC-peptide complexes. In contrast, naive CD4+ T cells polarized their cytoskeletal proteins in the absence of Ag. DC cytoskeletal rearrangement occurred at the same threshold of peptide-MHC complexes as that required for T cell activation. Furthermore, T cell activation was inhibited by specific blockade of DC cytoskeletal rearrangement. When TCR-MHC interaction was bypassed by using Con A-activated T cells, DC polarization was abrogated. In addition, directional ligation of MHC class II resulted in DC cytoskeletal polarization. Our findings suggest that a high Ag specificity is required for DC IS formation and that MHC class II signaling plays a central role in this process.

Langue d'origineEnglish
Pages (de-à)4479-4483
Nombre de pages5
JournalJournal of Immunology
Volume171
Numéro de publication9
DOI
Statut de publicationPublished - oct. 1 2003

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Immunology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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