TY - JOUR
T1 - Cutting Edge
T2 - Dendritic Cell Actin Cytoskeletal Polarization during Immunological Synapse Formation Is Highly Antigen-Dependent
AU - Al-Alwan, Monther M.
AU - Liwski, Robert S.
AU - Haeryfar, S. M.Mansour
AU - Baldridge, William H.
AU - Hoskin, David W.
AU - Rowden, Geoffrey
AU - West, Kenneth A.
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Dendritic cells (DC) actively rearrange their actin cytoskeleton to participate information of the immunological synapse (IS). In this study, we evaluated the requirements for DC participation in the IS. DC rearrange their actin cytoskeleton toward naive CD4+ T cells only in the presence of specific MHC-peptide complexes. In contrast, naive CD4+ T cells polarized their cytoskeletal proteins in the absence of Ag. DC cytoskeletal rearrangement occurred at the same threshold of peptide-MHC complexes as that required for T cell activation. Furthermore, T cell activation was inhibited by specific blockade of DC cytoskeletal rearrangement. When TCR-MHC interaction was bypassed by using Con A-activated T cells, DC polarization was abrogated. In addition, directional ligation of MHC class II resulted in DC cytoskeletal polarization. Our findings suggest that a high Ag specificity is required for DC IS formation and that MHC class II signaling plays a central role in this process.
AB - Dendritic cells (DC) actively rearrange their actin cytoskeleton to participate information of the immunological synapse (IS). In this study, we evaluated the requirements for DC participation in the IS. DC rearrange their actin cytoskeleton toward naive CD4+ T cells only in the presence of specific MHC-peptide complexes. In contrast, naive CD4+ T cells polarized their cytoskeletal proteins in the absence of Ag. DC cytoskeletal rearrangement occurred at the same threshold of peptide-MHC complexes as that required for T cell activation. Furthermore, T cell activation was inhibited by specific blockade of DC cytoskeletal rearrangement. When TCR-MHC interaction was bypassed by using Con A-activated T cells, DC polarization was abrogated. In addition, directional ligation of MHC class II resulted in DC cytoskeletal polarization. Our findings suggest that a high Ag specificity is required for DC IS formation and that MHC class II signaling plays a central role in this process.
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U2 - 10.4049/jimmunol.171.9.4479
DO - 10.4049/jimmunol.171.9.4479
M3 - Article
C2 - 14568920
AN - SCOPUS:0142242134
SN - 0022-1767
VL - 171
SP - 4479
EP - 4483
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -