Cyanidin-3-O-Glucoside-Rich Haskap Berry Administration Suppresses Carcinogen-Induced Lung Tumorigenesis in A/JCr Mice

Madumani Amararathna, David W. Hoskin, H. P.Vasantha Rupasinghe

Résultat de recherche: Articleexamen par les pairs

24 Citations (Scopus)

Résumé

In our previous study, we demonstrated that cyanidin-3-O-glucoside (C3G)-rich haskap (Lonicera caerulea L.) berry extracts can attenuate the carcinogen-induced DNA damage in normal lung epithelial cells in vitro. Here, the efficacy of lyophilized powder of whole haskap berry (C3G-HB) in lowering tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, (NNK)-induced lung tumorigenesis in A/JCr mice was investigated. Three weeks after daily oral administration of C3G-HB (6 mg of C3G in 0.2 g of C3G-HB/mouse/day), lung tumors were initiated by a single intraperitoneal injection of NNK. Dietary C3G-HB supplementation was continued, and 22 weeks later, mice were euthanized. Lung tumors were visualized through positron emission tomography (PET) and magnetic resonance imaging (MRI) 19 weeks after NNK injection. Dietary supplementation of C3G-HB significantly reduced the NNK-induced lung tumor multiplicity and tumor area but did not affect tumor incidence. Immunohistochemical analysis showed reduced expression of proliferative cell nuclear antigen (PCNA) and Ki-67 in lung tissues. Therefore, C3G-HB has the potential to reduce the lung tumorigenesis, and to be used as a source for developing dietary supplements or nutraceuticals for reducing the risk of lung cancer among high-risk populations.

Langue d'origineEnglish
Numéro d'article3823
JournalMolecules
Volume25
Numéro de publication17
DOI
Statut de publicationPublished - sept. 2020

Note bibliographique

Funding Information:
Funding: This study was supported by the Discovery Grant of Natural Sciences and Engineering Research Council (NSERC) of Canada (HPVR; Grant number RGPIN2016-05369) and the Cancer Research Training Program of the Beatrice Hunter Cancer Research Institute (BHCRI) supported by the Saunders-Matthey cancer prevention foundation (MA; Grant number CRTP2018). APC was sponsored by MDPI.

Publisher Copyright:
© 2020 by the authors.

ASJC Scopus Subject Areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

PubMed: MeSH publication types

  • Journal Article

Empreinte numérique

Plonger dans les sujets de recherche 'Cyanidin-3-O-Glucoside-Rich Haskap Berry Administration Suppresses Carcinogen-Induced Lung Tumorigenesis in A/JCr Mice'. Ensemble, ils forment une empreinte numérique unique.

Citer