Résumé
In our previous study, we demonstrated that cyanidin-3-O-glucoside (C3G)-rich haskap (Lonicera caerulea L.) berry extracts can attenuate the carcinogen-induced DNA damage in normal lung epithelial cells in vitro. Here, the efficacy of lyophilized powder of whole haskap berry (C3G-HB) in lowering tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, (NNK)-induced lung tumorigenesis in A/JCr mice was investigated. Three weeks after daily oral administration of C3G-HB (6 mg of C3G in 0.2 g of C3G-HB/mouse/day), lung tumors were initiated by a single intraperitoneal injection of NNK. Dietary C3G-HB supplementation was continued, and 22 weeks later, mice were euthanized. Lung tumors were visualized through positron emission tomography (PET) and magnetic resonance imaging (MRI) 19 weeks after NNK injection. Dietary supplementation of C3G-HB significantly reduced the NNK-induced lung tumor multiplicity and tumor area but did not affect tumor incidence. Immunohistochemical analysis showed reduced expression of proliferative cell nuclear antigen (PCNA) and Ki-67 in lung tissues. Therefore, C3G-HB has the potential to reduce the lung tumorigenesis, and to be used as a source for developing dietary supplements or nutraceuticals for reducing the risk of lung cancer among high-risk populations.
Langue d'origine | English |
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Numéro d'article | 3823 |
Journal | Molecules |
Volume | 25 |
Numéro de publication | 17 |
DOI | |
Statut de publication | Published - sept. 2020 |
Note bibliographique
Funding Information:Funding: This study was supported by the Discovery Grant of Natural Sciences and Engineering Research Council (NSERC) of Canada (HPVR; Grant number RGPIN2016-05369) and the Cancer Research Training Program of the Beatrice Hunter Cancer Research Institute (BHCRI) supported by the Saunders-Matthey cancer prevention foundation (MA; Grant number CRTP2018). APC was sponsored by MDPI.
Publisher Copyright:
© 2020 by the authors.
ASJC Scopus Subject Areas
- Analytical Chemistry
- Chemistry (miscellaneous)
- Molecular Medicine
- Pharmaceutical Science
- Drug Discovery
- Physical and Theoretical Chemistry
- Organic Chemistry
PubMed: MeSH publication types
- Journal Article