De novo infection and propagation of wild-type Hepatitis C virus in human T lymphocytes in vitro

Sonya A. MacParland, Tram N.O. Pham, Shashi A. Gujar, Tomasz I. Michalak

Résultat de recherche: Articleexamen par les pairs

41 Citations (Scopus)

Résumé

While exploring previous findings that ex vivo treatment of lymphoid cells from Hepatitis C virus (HCV)-infected individuals with T cell-stimulating mitogens augments detection of the residing virus, an in vitro HCV replication system was established, in which mitogen-induced T cell-enriched cultures served as HCV targets and the derived T cells multiplied virus during repeated serial passage. HCV replication was ascertained by detecting HCV RNA positive and negative strands, HCV NS5a and E2 proteins, release of HCV virions and nucleocapsids (confirmed by immunoelectron microscopy) and de novo infection of mitogen-induced T cells prepared from healthy donors. Further, affinity-purified normal human T lymphocytes were also susceptible to HCV infection in vitro and HCV replication was detected in pure T cells isolated from a patient with chronic hepatitis C. These results document that T cells can support propagation of HCV both in vivo and in vitro. The infection system established offers a valuable tool for in vitro studies on the entire cycle of HCV replication, virus cytopathogenicity and evaluation of antiviral agents against wild-type HCV in the natural host-cell milieu.

Langue d'origineEnglish
Pages (de-à)3577-3586
Nombre de pages10
JournalJournal of General Virology
Volume87
Numéro de publication12
DOI
Statut de publicationPublished - déc. 2006
Publié à l'externeOui

ASJC Scopus Subject Areas

  • Virology

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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