Detection of helicobacter pylori microevolution and multiple infection from gastric biopsies by housekeeping gene amplicon sequencing

Montserrat Palau, Núria Piqué, André M. Comeau, Gavin M. Douglas, M. José Ramírez-Lázaro, Sergio Lario, Xavier Calvet, Morgan G.I. Langille, David Miñana-Galbis

Résultat de recherche: Articleexamen par les pairs

3 Citations (Scopus)

Résumé

Despite the great efforts devoted to research on Helicobacter pylori, the prevalence of single-strain infection or H. pylori mixed infection and its implications in the mode of transmission of this bacterium are still controversial. In this study, we explored the usefulness of housekeeping gene amplicon sequencing in the detection of H. pylori microevolution and multiple infections. DNA was extracted from five gastric biopsies from four patients infected with distinct histopathological diagnoses. PCR amplification of six H. pylori-specific housekeeping genes was then assessed on each sample. Optimal results were obtained for the cgt and luxS genes, which were selected for amplicon sequencing. A total of 11,833 cgt and 403 luxS amplicon sequences were obtained, 2042 and 112 of which were unique sequences, respectively. All cgt and luxS sequences were clustered at 97% to 9 and 13 operational taxonomic units (OTUs), respectively. For each sample from a different patient, a single OTU comprised the majority of sequences in both genes, but more than one OTU was detected in all samples. These results suggest that multiple infections with a predominant strain together with other minority strains are the main way by which H. pylori colonizes the human stomach.

Langue d'origineEnglish
Numéro d'article97
JournalPathogens
Volume9
Numéro de publication2
DOI
Statut de publicationPublished - févr. 2020

Note bibliographique

Funding Information:
Funding: This research was funded by philanthropic donations from the FUNDACIÓ LA MARATÓ DE TV3 (Barcelona, Catalonia, Spain), grant number 1007/C/2013.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

ASJC Scopus Subject Areas

  • Immunology and Allergy
  • Molecular Biology
  • General Immunology and Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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