Effect of conjugated linoleic acid isomers on lipoproteins and atherosclerosis in the Syrian Golden hamster

Patricia L. Mitchell; Morgan A. Langille; Deborah L. Currie; Roger S. McLeod

doi:10.1016/j.bbalip.2005.04.007

Effect of conjugated linoleic acid isomers on lipoproteins and atherosclerosis in the Syrian Golden hamster

Patricia L. Mitchell, Morgan A. Langille, Deborah L. Currie, Roger S. McLeod

Medicine

Medicine

Résultat de recherche: Article › examen par les pairs

59 Citations (Scopus)

Résumé

Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA, C18:2 cis-9, cis-12) that are reported to have important biological activities, including protection against atherosclerosis. In this study, the potential role of the individual cis-9, trans-11 and trans-10, cis-12 isomers of CLA in atherogenesis were compared with LA in the Syrian Golden hamster. Supplementation of a high-fat, high-cholesterol diet (HFHC) with 1% (w/w) cis-9, trans-11 CLA or trans-10, cis-12 CLA did not significantly affect plasma cholesterol levels compared to supplementation with 1% (w/w) LA. Very low density lipoprotein cholesterol (VLDL-C) was lower and plasma triglycerides (TG) were higher in diets where C18:2 fatty acid was added to the HFHC diet, but neither the cis-9, trans-11 CLA group nor trans-10, cis-12 CLA group was significantly different from the LA control group. CLA supplementation did not significantly affect low density lipoprotein cholesterol (LDL-C). Trans-10, cis-12 CLA increased high density lipoprotein cholesterol (HDL-C) levels compared to LA or cis-9, trans-11 CLA (P < 0.02), and although the ratio of non-HDL-C:HDL-C in the cis-9, trans-11 CLA group (1.11 ± 0.54) and the trans-10, cis-12 CLA group (1.11 ± 0.21) was lower than the LA group (1.29 ± 0.45), the reduction did not reach statistical significance. Atherosclerosis was assessed in the ascending aorta by measuring the number of aortic cross-sections containing Oil Red O-stained intimal lesions. Compared to the LA group (60 ± 11%), both the cis-9, trans-11 CLA group (38 ± 8%) and the trans-10, cis-12 CLA group (28 ± 7%) had fewer sections displaying a fatty streak lesion, although the differences did not reach statistical significance. These results suggest that individual CLA isomers may reduce atherosclerotic lesion development in the hamster, but when compared to LA, the apparent atheroprotective effects do not correlate with beneficial changes in lipoprotein profile.

Langue d'origine	English
Pages (de-à)	269-276
Nombre de pages	8
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	1734
Numéro de publication	3
DOI	https://doi.org/10.1016/j.bbalip.2005.04.007
Statut de publication	Published - juin 1 2005

Note bibliographique

Funding Information:
The authors acknowledge the expert guidance of Dr. W. R. Currie, Ms. Brenda Ross and Mr. Steven Whitefield of the Department of Anatomy and Cell Biology during the assessment of atherosclerosis. We also thank Jessica Miller for a critical reading of the manuscript. This work was supported by grants (to R.S.M.) from Dairy Farmers of Canada and the Canadian Institutes of Health Research.

ASJC Scopus Subject Areas

Molecular Biology
Cell Biology

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10.1016/j.bbalip.2005.04.007

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title = "Effect of conjugated linoleic acid isomers on lipoproteins and atherosclerosis in the Syrian Golden hamster",

abstract = "Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA, C18:2 cis-9, cis-12) that are reported to have important biological activities, including protection against atherosclerosis. In this study, the potential role of the individual cis-9, trans-11 and trans-10, cis-12 isomers of CLA in atherogenesis were compared with LA in the Syrian Golden hamster. Supplementation of a high-fat, high-cholesterol diet (HFHC) with 1% (w/w) cis-9, trans-11 CLA or trans-10, cis-12 CLA did not significantly affect plasma cholesterol levels compared to supplementation with 1% (w/w) LA. Very low density lipoprotein cholesterol (VLDL-C) was lower and plasma triglycerides (TG) were higher in diets where C18:2 fatty acid was added to the HFHC diet, but neither the cis-9, trans-11 CLA group nor trans-10, cis-12 CLA group was significantly different from the LA control group. CLA supplementation did not significantly affect low density lipoprotein cholesterol (LDL-C). Trans-10, cis-12 CLA increased high density lipoprotein cholesterol (HDL-C) levels compared to LA or cis-9, trans-11 CLA (P < 0.02), and although the ratio of non-HDL-C:HDL-C in the cis-9, trans-11 CLA group (1.11 ± 0.54) and the trans-10, cis-12 CLA group (1.11 ± 0.21) was lower than the LA group (1.29 ± 0.45), the reduction did not reach statistical significance. Atherosclerosis was assessed in the ascending aorta by measuring the number of aortic cross-sections containing Oil Red O-stained intimal lesions. Compared to the LA group (60 ± 11%), both the cis-9, trans-11 CLA group (38 ± 8%) and the trans-10, cis-12 CLA group (28 ± 7%) had fewer sections displaying a fatty streak lesion, although the differences did not reach statistical significance. These results suggest that individual CLA isomers may reduce atherosclerotic lesion development in the hamster, but when compared to LA, the apparent atheroprotective effects do not correlate with beneficial changes in lipoprotein profile.",

author = "Mitchell, {Patricia L.} and Langille, {Morgan A.} and Currie, {Deborah L.} and McLeod, {Roger S.}",

note = "Funding Information: The authors acknowledge the expert guidance of Dr. W. R. Currie, Ms. Brenda Ross and Mr. Steven Whitefield of the Department of Anatomy and Cell Biology during the assessment of atherosclerosis. We also thank Jessica Miller for a critical reading of the manuscript. This work was supported by grants (to R.S.M.) from Dairy Farmers of Canada and the Canadian Institutes of Health Research.",

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doi = "10.1016/j.bbalip.2005.04.007",

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AU - Mitchell, Patricia L.

AU - Langille, Morgan A.

AU - Currie, Deborah L.

AU - McLeod, Roger S.

N1 - Funding Information: The authors acknowledge the expert guidance of Dr. W. R. Currie, Ms. Brenda Ross and Mr. Steven Whitefield of the Department of Anatomy and Cell Biology during the assessment of atherosclerosis. We also thank Jessica Miller for a critical reading of the manuscript. This work was supported by grants (to R.S.M.) from Dairy Farmers of Canada and the Canadian Institutes of Health Research.

PY - 2005/6/1

Y1 - 2005/6/1

N2 - Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA, C18:2 cis-9, cis-12) that are reported to have important biological activities, including protection against atherosclerosis. In this study, the potential role of the individual cis-9, trans-11 and trans-10, cis-12 isomers of CLA in atherogenesis were compared with LA in the Syrian Golden hamster. Supplementation of a high-fat, high-cholesterol diet (HFHC) with 1% (w/w) cis-9, trans-11 CLA or trans-10, cis-12 CLA did not significantly affect plasma cholesterol levels compared to supplementation with 1% (w/w) LA. Very low density lipoprotein cholesterol (VLDL-C) was lower and plasma triglycerides (TG) were higher in diets where C18:2 fatty acid was added to the HFHC diet, but neither the cis-9, trans-11 CLA group nor trans-10, cis-12 CLA group was significantly different from the LA control group. CLA supplementation did not significantly affect low density lipoprotein cholesterol (LDL-C). Trans-10, cis-12 CLA increased high density lipoprotein cholesterol (HDL-C) levels compared to LA or cis-9, trans-11 CLA (P < 0.02), and although the ratio of non-HDL-C:HDL-C in the cis-9, trans-11 CLA group (1.11 ± 0.54) and the trans-10, cis-12 CLA group (1.11 ± 0.21) was lower than the LA group (1.29 ± 0.45), the reduction did not reach statistical significance. Atherosclerosis was assessed in the ascending aorta by measuring the number of aortic cross-sections containing Oil Red O-stained intimal lesions. Compared to the LA group (60 ± 11%), both the cis-9, trans-11 CLA group (38 ± 8%) and the trans-10, cis-12 CLA group (28 ± 7%) had fewer sections displaying a fatty streak lesion, although the differences did not reach statistical significance. These results suggest that individual CLA isomers may reduce atherosclerotic lesion development in the hamster, but when compared to LA, the apparent atheroprotective effects do not correlate with beneficial changes in lipoprotein profile.

AB - Conjugated linoleic acid (CLA) is a group of positional and geometric isomers of linoleic acid (LA, C18:2 cis-9, cis-12) that are reported to have important biological activities, including protection against atherosclerosis. In this study, the potential role of the individual cis-9, trans-11 and trans-10, cis-12 isomers of CLA in atherogenesis were compared with LA in the Syrian Golden hamster. Supplementation of a high-fat, high-cholesterol diet (HFHC) with 1% (w/w) cis-9, trans-11 CLA or trans-10, cis-12 CLA did not significantly affect plasma cholesterol levels compared to supplementation with 1% (w/w) LA. Very low density lipoprotein cholesterol (VLDL-C) was lower and plasma triglycerides (TG) were higher in diets where C18:2 fatty acid was added to the HFHC diet, but neither the cis-9, trans-11 CLA group nor trans-10, cis-12 CLA group was significantly different from the LA control group. CLA supplementation did not significantly affect low density lipoprotein cholesterol (LDL-C). Trans-10, cis-12 CLA increased high density lipoprotein cholesterol (HDL-C) levels compared to LA or cis-9, trans-11 CLA (P < 0.02), and although the ratio of non-HDL-C:HDL-C in the cis-9, trans-11 CLA group (1.11 ± 0.54) and the trans-10, cis-12 CLA group (1.11 ± 0.21) was lower than the LA group (1.29 ± 0.45), the reduction did not reach statistical significance. Atherosclerosis was assessed in the ascending aorta by measuring the number of aortic cross-sections containing Oil Red O-stained intimal lesions. Compared to the LA group (60 ± 11%), both the cis-9, trans-11 CLA group (38 ± 8%) and the trans-10, cis-12 CLA group (28 ± 7%) had fewer sections displaying a fatty streak lesion, although the differences did not reach statistical significance. These results suggest that individual CLA isomers may reduce atherosclerotic lesion development in the hamster, but when compared to LA, the apparent atheroprotective effects do not correlate with beneficial changes in lipoprotein profile.

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Effect of conjugated linoleic acid isomers on lipoproteins and atherosclerosis in the Syrian Golden hamster

Résumé

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