Evaluation of the neutral red assay as a stress response indicator in cultivated mussels (Mytilus spp.) in relation to post-harvest processing activities and storage conditions

Joanne M. Harding, Cyr Couturier, G. Jay Parsons, Neil W. Ross

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45 Citations (Scopus)

Résumé

The neutral red assay (NRA) was evaluated and used as a biochemical indicator of stress response in cultured mussels that were subjected to various post-harvest processing and storage conditions. The NRA, which measures retention time of neutral red dye in the hemocyte organelle, the lysosome, can be correlated to the condition of mussels under various circumstances. Shelf life also provides an index of mussel condition and quality. The objectives of this study were to evaluate mussel stress response in relation to post-harvest conditions of (1) handling, (2) processing, and (3) storage practices. Neutral red retention was altered in mussels that underwent washing and declumping compared to unprocessed mussels. However, the process of debyssing substantially decreased neutral red retention in mussels. Mussels held under chilled or iced storage conditions displayed decreased neutral red retention compared to those held under wet storage. The results demonstrated that NRA was a useful index of physiological stress response in mussels subjected to conditions under various culture practices. The implications of this study for growers are that the practice of reimmersion of mussels for 24 h, especially after debyssing, and storage of mussels in water or on ice can reduce stress response and provide a better quality product with longer shelf life.

Langue d'origineEnglish
Pages (de-à)315-326
Nombre de pages12
JournalAquaculture
Volume231
Numéro de publication1-4
DOI
Statut de publicationPublished - mars 5 2004
Publié à l'externeOui

Note bibliographique

Funding Information:
We would like to thank AquaNet, the Canadian Networks Centres of Excellence in Aquaculture for partial funding of this research, School of Graduate Studies of Memorial University of Newfoundland, Fisheries and Marine Institute, Canadian Centre for Fisheries Innovation, and Black Gold for their generosity and support.

ASJC Scopus Subject Areas

  • Aquatic Science

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